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'Fluorescence Quenching' in keywords Facet   section ZfN Section C  [X]
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1976 (1)
1Author    Bernd Schmidt, HansJ. RurainskiRequires cookie*
 Title    Light-Dependent Interactions of Phenazine Methosulfate with 3-(3,4-Dichlorophenyl)-l,l-dimethylurea-Poisoned Chloroplasts  
 Abstract    The chlorophyll fluorescence of isolated chloroplasts in the presence of phenazine methosulfate (PMS) and 3-(3,4-dichlorophenyl)-l,l-dimethylurea (DCMU) can be quenched in a light-dependent reaction. This phenomenan has been studied and the following observations were made: 1. Quenching occurs under non-phosphorylating conditions and is stimulated by Mg2+ ions. 2. Under the same conditions, a light-dependent, Mg2+ stimulated transient decrease of absorp­ tion at 388 nm is observed which shows the spectral characteristics of PMS. 3. PMS is reversibly bound to chloroplasts. Under the experimental conditions used, binding amounts to as much as 0.5 mol PMS/mol chlorophyll. 4. Some uncouplers of photophosphorylation such as carbonylcyanide-m-chlorophenylhydrazon (CCCP) and atebrin analog abolish quenching, transient absorption change and binding of PMS. Others, such as methylamine, ammonia, gramicidin and nigericin do not. It is suggested that fluorescence quenching, transient absorption change and binding of PMS are causally related. The concept, postulated by others, that a high-energy state of the chloroplast membrane is involved in the fluorescence lowering 
  Reference    (Z. Naturforsch. 31c, 722 [1976]; received August 10 1976) 
  Published    1976 
  Keywords    Chlorophyll Fluorescence, Chloroplasts, Fluorescence Quenching, Energized Membrane 
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 TEI-XML for    default:Reihe_C/31/ZNC-1976-31c-0722.pdf 
 Identifier    ZNC-1976-31c-0722 
 Volume    31