| | 1 | Author
| | Requires cookie* | | | Title
| Pierre Mermier  | | | | Abstract
| Association The competition dialysis is based on the competition for a ligand between a macromolecule and an excess of another molecule able to form a diffusible complex with this ligand. The procedure used the Colowick's cell and is proposed for determining association constants of complexes which contain tightly bound or labile apoproteins. | | | |
Reference
| (Z. Naturforsch. 30c, 534 [1975]; received May 14 1975) | | | |
Published
| 1975 | | | |
Keywords
| Constant, Complex, Metalloprotein, Flow Dialysis | | | |
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| | | | | TEI-XML for
| default:Reihe_C/30/ZNC-1975-30c-0534_n.pdf | | | | Identifier
| ZNC-1975-30c-0534_n | | | | Volume
| 30 | |
| 2 | Author
| PierreM. Ermier, Wilhelm Hasselbach | Requires cookie* | | | Title
| The Effect of Calcium and Phosphate on the Biphasic Calcium Uptake by the Sarcoplasmic Reticulum | | | | Abstract
| The amplitude of the fast uptake and the initial rate of the slow uptake increase with in creasing free calcium concentrations, up to 30 [xu. In that range, both processes are correlated to each other. At higher concentrations, the slow uptake is more inhibited than the fast uptake. The fast uptake shows a maximum amplitude which remains unchanged in the presence of phosphate. The slow uptake leads to a nearly complete depletion of the external calcium, and its rate is pro portional to the phosphate concentration, even at physiological range. The sarcoplasmic ATPase liberates inorganic phosphate and the slow uptake | | | |
Reference
| (Z. Naturforsch. 30c, 777 [1975]; received July 17 1975) | | | |
Published
| 1975 | | | |
Keywords
| Sarcoplasmic Reticulum, Calcium, Phosphate, Flow Dialysis | | | |
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| | | | | TEI-XML for
| default:Reihe_C/30/ZNC-1975-30c-0777.pdf | | | | Identifier
| ZNC-1975-30c-0777 | | | | Volume
| 30 | |
| 3 | Author
| Pierre Mermier, Wilhelm Hasselbach | Requires cookie* | | | Title
| The Biphasic Ca2+-Uptake by the Fragmented Sarcoplasmic Reticulum | | | | Abstract
| The non-equilibrium dialysis has been used for kinetic studies of ATP dependent calcium up take by the sarcoplasmic reticulum. The uptake displays two phases, which are defined as fast and slow uptake. The former is an exponential function of time, with a half-life time of approxi mately 15 —20 sec, the latter presents the characteristics of an autocatalytic reaction. The fast uptake is characterized by its amplitude, the slow uptake by its rate. Compared with the fast up take, the slow uptake requires higher amounts of Mg2+ or ATP, and is more sensitive to pH variations and aging. The reasons which formerly prevented the resolution of the second phase from the first are discussed. It is concluded that the fast uptake is not a simple binding reaction, and that the slow uptake is more sensitive to changes by the vesicular membrane. | | | |
Reference
| (Z. Naturforsch. 30c, 593 [1975]; received June 6 1975) | | | |
Published
| 1975 | | | |
Keywords
| Flow Dialysis, Active Calcium Transport, Calcium Storage, Sarcoplasmic Reticulum | | | |
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| | | | | TEI-XML for
| default:Reihe_C/30/ZNC-1975-30c-0593.pdf | | | | Identifier
| ZNC-1975-30c-0593 | | | | Volume
| 30 | |
|
