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1995 (1)
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1Author    Paul Miller, W. Alfried, A. Linden, Claudio NicoliniRequires cookie*
 Title    Physico-Chemical Studies of Isolated Chromatin Compared with in situ Chromatin after Partial Hepatectomy in the Rat  
 Abstract    Chromatin was isolated from rat liver cells at 0, 3, 5, 11, 18 and 24 h following partial hepa­ tectomy. Consistent with findings in cultured cells stimulated to proliferate, there was an increase in chromatin molar ellipticity measured at 276 nm, and a decrease in thermal stability 3 to 8 h after surgery. These events occured prior to the onset of DNA synthesis. These early changes be­ tween non-proliferating (G0) and proliferating (Gj) cells, as well as later chromatin conformational changes observed at S and G2 phases, mimic changes in template activity. Results with sheared and unsheared chromatin (both with in vitro and in vivo systems) prove that structural and functional changes can be caused by even the slightest shearing during chromatin preparation, suggesting the loss of native chromatin organization. To eliminate this problem, ex­ periments were also conducted using chromatin in situ. A flow cytometer (FCM) was used to study unfixed liver cell suspensions stained with ethidium bromide (EB). Fluorescence was mea­ sured in the green spectral range after addition of increasing amounts of EB. Experimental evidence is provided that the same alteration in chromatin conformation can be best detected using low molar ratios of EB per unit DNA due to greater fluorescence emission in Gj respect to G0 cells. These correlated studies demonstrate that the same changes controlling chromatin organization in situ are detected also in the tertiary-quaternary structure of "isolated" chromatin. These changes in chromatin conformation are macromolecular events related to cell proliferation both at the G0 —Gt and Gt —S transitions. 
  Reference    Z. Naturforsch. 34c, 442 (1979); received December 15 1978/February 19 1979 
  Published    1979 
  Keywords    Isolated Chromatin, Partial Hepatectomy, Molar Ellipticity, Thermal Stability, Flow Cytometry 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0442.pdf 
 Identifier    ZNC-1979-34c-0442 
 Volume    34 
2Author    Ingunn Ulrich, Wolfgang UlrichRequires cookie*
 Title    Flow Cytometric DNA-Analysis of Plant Protoplasts Stained with DAPI  
 Abstract    prepared from leaves of various higher plants were stained with the specific DNA fluorochrome DAPI and measured with a pulse cytophotometer. DNA distribution curves of protoplasts showed DNA histograms like assays of animal cells stained with the same dye solu­ tion. The percentage of fractions of cell populations in Go/Gr , S-and G2 + M-phase of the cell cycle could be calculated. A simple method of protoplasts preparation and of flow cytometric estimation of DAPI stained plant protoplasts is described. This method allows cytogenetic and cytokinetic studies of cells from plant material and may be used for fluorescence activated cell sorting. 
  Reference    Z. Naturforsch. 41c, 1052 (1986); received July 23 1986 
  Published    1986 
  Keywords    Flow Cytometry, Plant Protoplasts, Protoplasts Preparation, DNA, DAPI Protoplasts 
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 TEI-XML for    default:Reihe_C/41/ZNC-1986-41c-1052.pdf 
 Identifier    ZNC-1986-41c-1052 
 Volume    41 
3Author    FriedrichJ. OttoRequires cookie*
 Title    Assessment of Persisting Chromosome Aberrations by Flow Karyotyping of Cloned Chinese Hamster Cells  
 Abstract    A preparation, staining and measuring protocol for high resolution flow cytometry of chromo-somes was developed. This method allows us to identify all chromosome types and is suited for characterization of permanent cell lines and cell clones by establishing their flow karyotypes. In cell clones this procedure can be used for the detection of chromosomal aberrations which appear spontaneously or are induced by mutagen treatment and persist in the cell population. 
  Reference    Z. Naturforsch. 43c, 948—954 (1988); received May 2/July 18 1988 
  Published    1988 
  Keywords    Chinese Hamster Cells, Chromosomal Aberrations, Karyotyping, Flow Cytometry, Cytogenetics 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0948.pdf 
 Identifier    ZNC-1988-43c-0948 
 Volume    43 
4Author    TetsuyaM. AtsunoRequires cookie*
 Title    A New Clerodane Diterpenoid Isolated from Propolis  
 Abstract    A physiologically active substance has been isolated from Brazilian propolis and charac­ terized as a new clerodane diterpenoid, as indicated by human hepatocellular carcinoma HuH 13 cell cytotoxicity assays. This compound inhibited growth of the hepatom a cells at a concentration around 10 [ig/ml and arrested the tumor cells at S phase as revealed by flow cytometry. A t higher concentrations it exerted lethal damage. The compound showed cyto­ toxicity on human lung carcinoma HLC-2, HeLa, KB and rat W 3 Y cells, whereas human diploid foreskin and primary rabbit kidney cells were less affected. 
  Reference    Z. Naturforsch. 50c, 93—9 (1995); received August 5/October 18 1994 
  Published    1995 
  Keywords    Propolis, Clerodane D iterpenoid, Tumor Cells, Flow Cytometry, Colcemid, Cytotoxicity 
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 TEI-XML for    default:Reihe_C/50/ZNC-1995-50c-0093.pdf 
 Identifier    ZNC-1995-50c-0093 
 Volume    50