| 1 | Author | Maurice Jay, Vincenzo De Luca, Ragai Ibrahim | Requires cookie* |
| Title | Meta-Methylation o f Flavonol Rings A (8-) and B (3'-) Is Catalysed by Two Distinct O-Methyltransferases in Lotus corniculatus  | ||
| Abstract | Two O-methyltransferases specific for flavonol rings A and B were isolated from young flower buds of Lotus corniculatus. They were partially purified by ammonium sulphate precipitation and successive chromatography on Sephadex G-100 and Polybuffer ion exchanger. One enzyme focused at pi 5.5 and catalysed the O-methylation of position 8 of flavonols with a pH optimum of 8.1. The other enzyme had a pi o f 5.1 and preferentially attacked position 3' at an optimum pH of 7.7. The methylated products o f both enzymes seem to contribute to the flower colour of Lotus and may be used as biochemical markers in genetic studies of this genus. | ||
| Reference | Z. Naturforsch. 38c, 413 (1983); received December 21 1982 | ||
| Published | 1983 | ||
| Keywords | Lotus corniculatus, Leguminosae, O-Methyltransferase, Flavonols, 8-O-Methylation, 3'-0-Methylation | ||
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