Go toArchive
Browse byFacets
Bookbag ( 0 )
'Flavonoid 3 Hydroxylase' in keywords
Results  2 Items
Sorted by   
Section
Publication Year
1981 (1)
1980 (1)
1Author    G. Forkm, W. Heller, H. GrisebachRequires cookie*
 Title    Anthocyanin Biosynthesis in Flowers of Matthiola incana Flavanone 3-and Flavonoid 3'-Hydroxylases  
 Abstract    Enzyme preparations from flowers o f defined genotypes o f Matthiola incana contain two dif­ ferent hydroxylases for hydroxylation of naringenin in the 3-and 3'-position, respectively. The 3-hydroxylase is a soluble enzyme and requires as cofactors 2-oxoglutarate, Fe2+ and ascorbate. Besides naringenin eriodictyol is a substrate for the 3-hydroxylase. The 3'-hydroxylase is localized in the microsomal fraction and requires NADPH as cofactor. Naringenin and dihydro-kaempferol but not 4-coumarate or 4-coumaroyl-CoA are substrates for this enzyme. 3'-Hy-droxylase activity is present only in genetic lines of M. incana with the wild-type allele b +. 
  Reference    Z. Naturforsch. 35c, 691—6 (1980); received June 25 1980 
  Published    1980 
  Keywords    Anthocyanins, Flavonoids, Biosynthesis, Flavanon 3-Hydroxylase, Flavonoid 3'-Hydroxylase, Matthiola incana 
  Similar Items    Find
 DEBUG INFO      
 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0691.pdf 
 Identifier    ZNC-1980-35c-0691 
 Volume    35 
2Author    G. Forkmann, G. StotzRequires cookie*
 Title    Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3'-Hydroxylase Activity in Antirrhinum majus (Snapdragon)  
 Abstract    In flower extracts o f defined genotypes o f Antirrhinum majus, two different hydroxylases were found catalysing the hydroxylation o f naringenin and eriodictyol in the 3-position and of naringenin in the 3'-position. The 3-hydroxylase is a soluble enzyme and belongs according to its cofactor requirement to the 2-oxoglutarate-dependent dioxygenases. Investigations on different genotypes revealed a clear correlation between block o f the anthocyanin pathway by recessive alleles of the gene inc and a complete lack of 3-hydroxylase activity. Chemogenetic studies on different genotypes suggested that the 3'-hydroxyl group of the B-ring of flavonoids is introduced at the stage of C15 intermediates. The corresponding 3'-hydroxylase was found to be localized in the microsomal fraction and required NADPH as cofactor. In confirmation o f the chemogenetic studies, a strict correlation was found between 3'-hydroxylase activity and the gene eos which is known to control the hydroxylation o f flavones, flavonols and anthocyanins in the 3'-position. These results are similar to those previously obtained with Matthioia incana. 
  Reference    Z. Naturforsch. 36c, 411 (1981); received January 20 1981 
  Published    1981 
  Keywords    Anthocyanin Biosynthesis, Antirrhinum majus, Flavonoids, Flavanone 3-Hydroxylase, Flavonoid 3'-Hydroxylase, Genetic Control 
  Similar Items    Find
 DEBUG INFO      
 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0411.pdf 
 Identifier    ZNC-1981-36c-0411 
 Volume    36