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2000 (1)
1996 (1)
1Author    BlahoslavM. Aršálek, Renata RojíčkováRequires cookie*
 Title    Stress Factors Enhancing Production of Algal Exudates: a Potential Self-Protective Mechanism?  
 Abstract    Algae are known to produce extracellular organic substances under optimum conditions and increase their production under stress. The changes in amount and composition of extra­ cellular carbohydrates and proteins of three green algae Scenedesmus quadricauda, Chlorella kessleri and Raphidocelis subcapitata (known as Selenastrum capricornutum) were studied after a 5-days' cultivation under the influence of different types o f stress factors (osm otic, organic, and heavy metal stressors). NaCl enhanced the quantity of carbohydrates more than proteins. A higher increase o f proteins than carbohydrates was observed after addition of 3,5-dichlorophenol, glyphosate and cadmium chloride to algal cultures. The production of dissolved organic matter differs from species to species, with the age of a culture and the type of stressor. 
  Reference    Z. Naturforsch. 51c, 646 (1996); received May 28/June 25. 1996 
  Published    1996 
  Keywords    Algae, Extracellular Products, Proteins, Carbohydrates 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0646.pdf 
 Identifier    ZNC-1996-51c-0646 
 Volume    51 
2Author    Ferng-Ruey Chen, Ping-Chung Liu, Kuo-Kau LeeRequires cookie*
 Title    Lethal Attribute of Serine Protease Secreted by Vibrio alginolyticus Strains in Kuruma Prawn Penaeus japonicus  
 Abstract    Toxicity of the extracellular products (E C P) and the lethal attribute of serine protease secreted by five pathogenic Vibrio alginolyticus strains from various sources in kuruma prawn Penaeus japonicus were studied. The E C P s of organisms originally isolated from diseased kuruma prawn or small abalone Haliotis diversicolor supertexta were more lethal (L D 50 value of 0.48 or 0.41 ^ig protein/g prawn) than those from diseased tiger prawn P. m onodon, yellow-fin porgy Acanthopagrus latus or horse m ackerel (L D 50 value of 0 .9 8 -1 .1 7 ^ig protein/g prawn). All the EC Ps manifested strong, weak and no activities against gelatin, sheep eryth­ rocytes and chitin, respectively. In immunodiffusion tests using rabbit antiserum to a purified 33 kDa serine protease of strain Swy against E C P of each tested strain produced one single precipitation band in each treatm ent. Furtherm ore, the serine protease was suggested to be the dominant protease secreted by V. alginolyticus strains tested since the majority of enzy­ matic activity of the respective E C P was inhibited by phenylmethanesulfonyl fluoride (PM SF). A higher inhibition of serine protease activity by PM SF resulted in lower mortality rate of the EC Ps injected into the prawns suggesting that the protease is one of the major lethal factor(s) secreted by V alginolyticus. 
  Reference    Z. Naturforsch. 55c, 94—9 (2000); received Septem ber 9/Novem ber 8 1999 
  Published    2000 
  Keywords    Extracellular Products, Lethality, Penaeus japonicus, Serine Protease, Vibrio alginolyticus 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0094.pdf 
 Identifier    ZNC-2000-55c-0094 
 Volume    55