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1983 (2)
1Author    Akira Taketo, Yoriko TaketoRequires cookie*
 Title    Effect of Streptococcal Extracellular Nuclease on the Carrier Activity of RNA for Streptolysin S  
 Abstract    Upon digestion with a streptococcal extracellular nuclease, yeast RNA yielded acid-insoluble core having increased carrier activity for streptolysin S. The carrier activity was found in minor fractions of the core which were eluted from a DEAE-cellulose column at higher salt concentrations. Upon gel filtration through a Sephadex G-75 column, the effective component (Fr. I) was eluted earlier than bulk oligonucleotides (Fr. II). Nucleotide composition (in mol %) of Fr. I was AMP: 21.8; GMP: 55.1; CMP: 8.2; UMP: 14.9, whereas that o f Fr. II was AMP: 38.0; GMP: 33.1; CMP: 8.0; UMP: 20.9. Chromatographic patterns of SLS complex induced by Fr. I were similar to those of the toxin formed in the presence o f active fraction prepared from RNase I core. Hemolytic activity of the latter complex was, like the former, unaffected by streptococcal nuclease treatment. The carrier activity o f DNA digested with the nuclease was also investigated. 
  Reference    Z. Naturforsch. 38c, 107 (1983); received October 1 1982 
  Published    1983 
  Keywords    Streptolysin S, RNA Effect, Extracellular Nuclease, Streptococcus hemolyticus 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0107.pdf 
 Identifier    ZNC-1983-38c-0107 
 Volume    38 
2Author    Akira Taketo, Yoriko TaketoRequires cookie*
 Title    Decreased Production of RNA-Streptolysin S in Streptococci Devoid of Extracellular RNase Activity  
 Abstract    In cultures of certain streptococcal strains, R N A added as a carrier for streptolysin S (SLS) was hardly degraded, owing to deficiency of extracellular RNase activity. Production of RNA-SLS into culture supernatant was markedly reduced in the RNase-deficient streptococci. Even in these RNase-less strains, guanylic-acid rich oligonucleotides, polyG or trypan blue effectively induced SLS production, as in RNase-positive cells. These results demonstrate involvement of the streptococcal nuclease in manifestation of SLS-inducing effect of exogenous RNA. Additional data indicated that cellular growth was promoted by supplementation of RNA, in the nuclease-producing streptococci. 
  Reference    Z. Naturforsch. 38c, 635—639 (1983); received December 6 1982/March 1 1983 
  Published    1983 
  Keywords    Streptolysin S, Extracellular Nuclease, RN A Effect, Streptococcus hem olyticus 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0635.pdf 
 Identifier    ZNC-1983-38c-0635 
 Volume    38