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'Extracellular Acidification' in keywords Facet   section ZfN Section C:Volume 056  [X]
Facet   Publication Year 2001  [X]
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2001[X]
1Author    OliverO. Tte, Andreas Pachten, Frauke Hein, Wolfgang BarzRequires cookie*
 Title    Early Elicitor-Induced Events in Chickpea Cells: Functional Links between Oxidative Burst, Sequential Occurrence of Extracellular Alkalinisation and Acidification, K+/H + Exchange and Defence-Related Gene Activation  
 Abstract    Elicitation of cultured chickpea (Cicer arietinum L.) cells stimulates a signal transduction pathway leading to several rapid responses: (1) oxidative burst, (2) extracellular alkalinisa­ tion, (3) extracellular acidification, (4) transient K+ efflux, and (5) activation of defence related genes all within 2 hours. Induced genes are encoding acidic and basic chitinases, a thaumatin-like protein and isoflavone reductase. All these elicitor-induced responses are in­ hibited by the Ser/Thr protein kinase inhibitor staurosporine and the anion channel blocker anthracene-9-carboxylic acid but stimulated by the Ser/Thr protein phosphatase 2A inhibitor cantharidin. The oxidative burst leads to a transient extracellular H2 0 2 accumulation which seems to be preceded by 0 2~ production, indicating dismutation of 0 2~ to H2 0 2. The oxida­ tive burst is accompanied by transient alkalinisation of the culture medium which is followed by long-lasting extracellular acidification. An 80 percent inhibition of the alkalinisation after complete inhibition of the H2 0 2 burst with diphenylene iodonium indicates that the elicitor induced increase of extracellular pH is mainly based on a proton consumption for 0 2~ dismutation. A simultaneous deactivation of the plasma membrane H+-ATPase during oxida­ tive burst and extracellular alkalinisation is also suggested. The elicitor-stimulated extracellu­ lar acidification is inhibited by the plasma membrane H+-ATPase inhibitor N, N'-dicyclohex-ylcarbodiimide assuming a reactivation of the H+-ATPase 25 min after elicitation. Extracellular acidification seems not to be necessary for elicitor-induced activation of defence related genes. Opposite modulation of K+ and proton fluxes after elicitation and/or treatment with the H +-ATPase effectors fusicoccin or N, N'-dicyclohexylcarbodiimide indicate that the elicitor induced transient K+ efflux is regulated by a K+/H+ exchange reaction. 
  Reference    Z. Naturforsch. 56c, 65—76 (2001); received August 29 2000 
  Published    2001 
  Keywords    Cicer arietinum, Defence Related Gene Activation, Extracellular Acidification, Oxidative Burst 
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 TEI-XML for    default:Reihe_C/56/ZNC-2001-56c-0065.pdf 
 Identifier    ZNC-2001-56c-0065 
 Volume    56