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'Excited States' in keywords Facet   Publication Year 1974  [X]
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1974[X]
1Author    Wanda Wagner, Maria Zaborowska, Wlodzimierz Puzyna, David ShugarRequires cookie*
 Title    Studies on Purine-Pyrimidine Hydrogen Bonded Base Pairing by Means of Absorption and Emission Spectroscopy  
 Abstract    Procedures are described for measurement of association constants between potentially com­ plementary purine and pyrimidine bases by hydrogen bonding in non-aqueous medium by means of absorption and emission spectroscopy. The methods require that one of the two constituents be fluorescent and exhibit a long wavelength absorption band not, or only partially, overlapped by the second constituent. The foregoing has been applied to measurements of the association constants, and other parameters, of hydrogen-bonded complexes of 2-aminopurine with 1-substituted uracil, thymine and barbital in chloroform solution, both in the ground and excited states. It was estab­ lished that the 1:1 hydrogen-bonded base pairs 2-aminopurine : 1-cyclohexyluracil and 2-amino­ purine: 1-octylthymine maintain the ground-state equilibrium during the life time of the excited state, whereas for the corresponding pair 2-aminopurine: 1-methylbarbital this equilibrium is per­ turbed on excitation. The influence of N-alkylation of the 2-aminopurine residue on base-pairing with the pyrimidines has also been examined. The results are compared with those obtained by other methods. The specific advantages of the procedures employed, and their possible applications to studies on the behaviour of fluorescent residues in polynucleotides, are discussed. 
  Reference    (Z. Naturforsch. 29c, 672 [1974]; received August 1 1974) 
  Published    1974 
  Keywords    Purines and Pyrimidines, Hydrogen Bonding, Base-pairing, Excited States, Fluorescence 
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 TEI-XML for    default:Reihe_C/29/ZNC-1974-29c-0672.pdf 
 Identifier    ZNC-1974-29c-0672 
 Volume    29