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'Euglena gracilis' in keywords Facet   section ZfN Section C  [X]
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1Author    Edward Göbel, Ruth Riessner, Peter PohlRequires cookie*
 Title    Einfluß von DCMU auf die Bildung von Lipiden und Fettsäuren und auf die Ultrastruktur von Euglena gracilis Influence of DCMU on the Formation of Lipids and Fatty Acids, and on the Ultrastructure of Euglena gracilis  
 Abstract    Euglena g racilis Klebs (strain Z) was grown heterotrophically in the dark for 6 days. Sub­ sequently, the cells were grown photoautotrophically under white fluorescent light with varying amounts of 3-(3,4-dichlorophenyl)-l,ldimethyl urea (DCMU) (1 .7 X 1 0 -9 — 1 .0 X 1 0 -5 mol/1) added 
  Reference    (Z. Naturforsch. 31c, 687 [1976]; eingegangen am 9. März/20. September 1976) 
  Published    1976 
  Keywords    Euglena gracilis, Fatty Acids, Lipids, Ultrastructure, Chloroplasts, Mitochondria 
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 TEI-XML for    default:Reihe_C/31/ZNC-1976-31c-0687.pdf 
 Identifier    ZNC-1976-31c-0687 
 Volume    31 
2Author    ErichF. Elstner, W. OsswaldRequires cookie*
 Title    Chlorophyll Photobleaching and Ethane Production in Dichlorophenyldimethylurea-(DCMU) or Paraquat-Treated Euglena gracilis Cells  
 Abstract    Light dependent (35 Klux) chlorophyll bleaching in autotrophically grown Euglena gracilis cells at slightly acidic pH (6.5 —5.4) is stimulated by the photosystem II blockers DCMU and DBMIB (both 10~5 m) as well as by the autooxidizable photosystem I electron acceptor, paraquat (1 0 -3 m). Chlorophyll photobleaching is accompanied by the formation o f thiobarbituric acid — sensitive material ("malondialdehyde") and ethane. Both chlorophyll photobleaching and light dependent ethane formation are partially prevented by higher concentrations (10~* m) o f the autooxidizable photosystem II electron acceptor DBMIB or by sodium bicarbonate (25 m M). In vitro studies with cell free extracts (homogenates) from E. gracilis suggest that a-linolenic acid oxidation by excited (reaction center II) chlorophyll represents the driving force for both ethane formation and chlorophyll bleaching. Ethane formation thus appears to be a sensitive and non-destructive "in vivo'' marker for both restricted energy dissipation in photosystem II and, conditions yielding reactive oxygen species at the reducing side o f photosystem I. 
  Reference    Z. Naturforsch. 35c, 129—135 (1980); received August 20/September 28 1979 
  Published    1980 
  Keywords    Chlorophyll Bleaching, Herbicides, Euglena gracilis, Ethane, Fat Oxidation 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0129.pdf 
 Identifier    ZNC-1980-35c-0129 
 Volume    35 
3Author    F. Schuler, P. Brandt, W. WießnerRequires cookie*
 Title    Isolierung von PS II-Partikeln mit in vivo Eigenschaften aus Euglena gracilis, Stamm Z Isolation of PS II-Particels with in vivo Characteristics from Euglena gracilis, Stamm Z  
 Abstract    An improved method for isolation of (photosystem II)-particles from Euglena gracilis, strain Z was established. PS II-particles isolated by ultrasonic treatment and following differential centrifugation show fluorescence emission and absorption spectra identical with in vivo properties o f Euglena gracilis. These PS II-particles have only PS II-activity and contain CP a, the typical chlorophyll-protein-complex o f PS II. N o contamination o f PS I-components are detectable. 
  Reference    Z. Naturforsch. 37c, 256—259 (1982); eingegangen am 21. Dezember 1981 
  Published    1982 
  Keywords    Chlorophyll-Protein-Complex, Euglena gracilis, Photosystem II Particle 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0256.pdf 
 Identifier    ZNC-1982-37c-0256 
 Volume    37 
4Author    RitaM. Fink, ErichF. ElstnerRequires cookie*
 Title    Comparison of Different Methods for the Determination of Phenylalanine Hydroxylase Activity in Rat Liver and Euglena gracilis  
 Abstract    Three different methods for the determination of phenylalanine hydroxylase activity have been compared: a) Differential photometric assay of the increase in tyrosine concentration in the presence of phenylalanine; b) Product separation by thin layer chromatography and scintillation counting of the [14C]tyrosine formed; c) HPLC separation and spectrofluorometric quantification of derivatized amino acids. A comparison of the activities of phenylalanine hydroxylase in rat liver and Euglena gracilis clearly showed that only rat liver contains this enzymic activity as shown by methods b) and c) although pseudo-activity of Euglena gracilis preparations was found during the spectrophoto-metric test a). The HPLC method proved to be the fastest, most reliable and convenient method for direct tyrosine determination and thus for measuring phenylalanine hydroxylase activity. 
  Reference    Z. Naturforsch. 39c, 728—733 (1984); received January 27/April 14 1984 
  Published    1984 
  Keywords    Phenylalanine Hydroxylase, Rat Liver, Euglena gracilis, Tyrosine Determination 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0728.pdf 
 Identifier    ZNC-1984-39c-0728 
 Volume    39 
5Author    T. Kreuels, R. Joerres, W. M. Artin, K. BrinkmannRequires cookie*
 Title    System Analysis of the Circadian Rhythm of Euglena gracilis, II: Masking Effects and Mutual Interactions of Light and Temperature Responses  
 Abstract    Motility o f Euglena gracilis shows free running circadian rhythms. The circadian system is sensitive to light and temperature signals, but it is always m asked by direct responses o f m otility to light (photokinesis) and temperature (therm okinesis). By means o f a com partim ental m odel which defines the interrelations between the pathways o f therm okinesis, photokinesis and the circadian system a unifying view o f effects o f temperature and light input signals is outlined. According to the model, and using double sine input signals the dynam ics o f thermokinesis is described by a differential am plifier with constant gain. Although thermokinesis heavily masks circadian responses to tem perature signals, the lim ited range o f circadian entrainment is indirectly dem onstrated by a lim ited reappearance o f free running circadian oscillations after stopping the tem perature program. Free running circadian oscillations do reappear only after pretreatment with tem perature periods near the circadian eigenperiod. A white mutant lacking photosynthesis is used to investigate the role o f photosynthesis in the signal processing. Although light synchronizes the circadian rhythms o f the white mutant if applied as single input, it does not affect the m otility if applied together with temperature inputs near the circadian eigenperiod. These results indicate frequency dependent m utual interactions between the model compartments. 
  Reference    Z. Naturforsch. 39c, 801—8 (1984); received February 2 2 /April 27 1984 
  Published    1984 
  Keywords    Circadian Rhythm, System Analysis, Euglena gracilis, Masking Temperature Responses 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0801.pdf 
 Identifier    ZNC-1984-39c-0801 
 Volume    39 
6Author    M. V. Rossetti, A. A. Juknat, A. M. Del, C. BatlleRequires cookie*
 Title    Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis  
 Abstract    A highly efficient m ethod em ploying NaSCN as a chaotropic agent was used to dissociate the m embrane-bound porphobilinogen-deam inase. T he sam e sequence o f steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deam inase. The chromatographic behaviour o f both proteins was quite similar. Euglena gracilis deam inase appears to exist in an equilibrium mixture o f two active species of relative molecular m asses o f 40000 and 20000. 
  Reference    Z. Naturforsch. 44c, 578 (1989); received July 25 1988/April 4 1989 
  Published    1989 
  Keywords    Euglena gracilis, P orphobilinogen-D eam inase, Porphyrins, Soluble, Particulate 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0578.pdf 
 Identifier    ZNC-1989-44c-0578 
 Volume    44 
7Author    A. Kiinnea, E. Pistoriusb, K. Kloppstechc, E. De, G. RootdRequires cookie*
 Title    Circadian Synthesis of Light-Harvesting-Chlorophyll-Proteins in Euglena gracilis Is under Translational Control  
 Abstract    Two proteins with apparent molecular masses of 17 and 24 kD that are synthesized in a circadian manner in the phytoflagellate Euglena gracilis, were recognized as proteins belong­ ing to the family of light-harvesting-chlorophyll-proteins (LHCPs) of class 1(17 kD) and of class II (24 kD). Identification was achieved by N-terminal sequencing of the proteins iso­ lated from two-dimensional polyacrylamide gels and by detection with an anti-LHCP II se­ rum. While it was found that the total am ount of LHCPs remains almost constant, when Euglena is grown under diurnal conditions (12 h light and 12 h dark), we could show that the amount of newly synthesized 17 and 24 kD proteins varies about 20-fold with a maximum of synthesis in the light phase. In contrast, the analysis of the mRNA levels at different times revealed only minor differences in the stationary concentration of the LHCP specific mRNA, indicating that the control of LHCP synthesis is at the translational level. Principally, the same finding was obtained using inhibitors of transcription. Thus, it is concluded that the expression of LHCPs in Euglena gracilis in contrast to that of higher plants is primarily regulated at the translational level. 
  Reference    Z. Naturforsch. 53c, 1017—1026 (1998); received July 7/August 3 1998 
  Published    1998 
  Keywords    Euglena gracilis, Circadian Rhythm, Translational Control, Protein Synthesis, Light-Harvesting-Chlorophyll-Proteins 
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 TEI-XML for    default:Reihe_C/53/ZNC-1998-53c-1017.pdf 
 Identifier    ZNC-1998-53c-1017 
 Volume    53 
8Author    E. F. Elstner, Irene PilsRequires cookie*
 Title    Ethane Formation and Chlorophyll Bleaching in DCMU-Treated Euglena gracilis Cells and Isolated Spinach Chloroplast Lamellae  
 Abstract    Light dependent chlorophyll bleaching and ethane formation in Euglena gracilis cells at slightly acidic pH values above 5.2 are enhanced by DCMU and other photosystem II blockers. Excited chlorophyll under conditions of limited energy dissipation seems to be the oxidant for co-3 unsaturated fatty acids, yielding ethane and other (ethylene, "malondialdehyde") oxidation products, cooxidizing chlorophyll. 
  Reference    Z. Naturforsch. 34c, 1040—1043 (1979) 
  Published    1979 
  Keywords    received Chlorophyll Bleaching, Ethane Formation, Euglena gracilis, Herbicidal Action, Lipid Peroxidation 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-1040.pdf 
 Identifier    ZNC-1979-34c-1040 
 Volume    34