| | 1 | Author
| Edward Göbel, Ruth Riessner, Peter Pohl | Requires cookie* | | | Title
| Einfluß von DCMU auf die Bildung von Lipiden und Fettsäuren und auf die Ultrastruktur von Euglena gracilis Influence of DCMU on the Formation of Lipids and Fatty Acids, and on the Ultrastructure of Euglena gracilis  | | | | Abstract
| Euglena g racilis Klebs (strain Z) was grown heterotrophically in the dark for 6 days. Sub sequently, the cells were grown photoautotrophically under white fluorescent light with varying amounts of 3-(3,4-dichlorophenyl)-l,ldimethyl urea (DCMU) (1 .7 X 1 0 -9 — 1 .0 X 1 0 -5 mol/1) added | | | |
Reference
| (Z. Naturforsch. 31c, 687 [1976]; eingegangen am 9. März/20. September 1976) | | | |
Published
| 1976 | | | |
Keywords
| Euglena gracilis, Fatty Acids, Lipids, Ultrastructure, Chloroplasts, Mitochondria | | | |
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| default:Reihe_C/31/ZNC-1976-31c-0687.pdf | | | | Identifier
| ZNC-1976-31c-0687 | | | | Volume
| 31 | |
| 2 | Author
| ErichF. Elstner, W. Osswald | Requires cookie* | | | Title
| Chlorophyll Photobleaching and Ethane Production in Dichlorophenyldimethylurea-(DCMU) or Paraquat-Treated Euglena gracilis Cells | | | | Abstract
| Light dependent (35 Klux) chlorophyll bleaching in autotrophically grown Euglena gracilis cells at slightly acidic pH (6.5 —5.4) is stimulated by the photosystem II blockers DCMU and DBMIB (both 10~5 m) as well as by the autooxidizable photosystem I electron acceptor, paraquat (1 0 -3 m). Chlorophyll photobleaching is accompanied by the formation o f thiobarbituric acid — sensitive material ("malondialdehyde") and ethane. Both chlorophyll photobleaching and light dependent ethane formation are partially prevented by higher concentrations (10~* m) o f the autooxidizable photosystem II electron acceptor DBMIB or by sodium bicarbonate (25 m M). In vitro studies with cell free extracts (homogenates) from E. gracilis suggest that a-linolenic acid oxidation by excited (reaction center II) chlorophyll represents the driving force for both ethane formation and chlorophyll bleaching. Ethane formation thus appears to be a sensitive and non-destructive "in vivo'' marker for both restricted energy dissipation in photosystem II and, conditions yielding reactive oxygen species at the reducing side o f photosystem I. | | | |
Reference
| Z. Naturforsch. 35c, 129—135 (1980); received August 20/September 28 1979 | | | |
Published
| 1980 | | | |
Keywords
| Chlorophyll Bleaching, Herbicides, Euglena gracilis, Ethane, Fat Oxidation | | | |
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| default:Reihe_C/35/ZNC-1980-35c-0129.pdf | | | | Identifier
| ZNC-1980-35c-0129 | | | | Volume
| 35 | |
| 4 | Author
| RitaM. Fink, ErichF. Elstner | Requires cookie* | | | Title
| Comparison of Different Methods for the Determination of Phenylalanine Hydroxylase Activity in Rat Liver and Euglena gracilis | | | | Abstract
| Three different methods for the determination of phenylalanine hydroxylase activity have been compared: a) Differential photometric assay of the increase in tyrosine concentration in the presence of phenylalanine; b) Product separation by thin layer chromatography and scintillation counting of the [14C]tyrosine formed; c) HPLC separation and spectrofluorometric quantification of derivatized amino acids. A comparison of the activities of phenylalanine hydroxylase in rat liver and Euglena gracilis clearly showed that only rat liver contains this enzymic activity as shown by methods b) and c) although pseudo-activity of Euglena gracilis preparations was found during the spectrophoto-metric test a). The HPLC method proved to be the fastest, most reliable and convenient method for direct tyrosine determination and thus for measuring phenylalanine hydroxylase activity. | | | |
Reference
| Z. Naturforsch. 39c, 728—733 (1984); received January 27/April 14 1984 | | | |
Published
| 1984 | | | |
Keywords
| Phenylalanine Hydroxylase, Rat Liver, Euglena gracilis, Tyrosine Determination | | | |
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| default:Reihe_C/39/ZNC-1984-39c-0728.pdf | | | | Identifier
| ZNC-1984-39c-0728 | | | | Volume
| 39 | |
| 5 | Author
| T. Kreuels, R. Joerres, W. M. Artin, K. Brinkmann | Requires cookie* | | | Title
| System Analysis of the Circadian Rhythm of Euglena gracilis, II: Masking Effects and Mutual Interactions of Light and Temperature Responses | | | | Abstract
| Motility o f Euglena gracilis shows free running circadian rhythms. The circadian system is sensitive to light and temperature signals, but it is always m asked by direct responses o f m otility to light (photokinesis) and temperature (therm okinesis). By means o f a com partim ental m odel which defines the interrelations between the pathways o f therm okinesis, photokinesis and the circadian system a unifying view o f effects o f temperature and light input signals is outlined. According to the model, and using double sine input signals the dynam ics o f thermokinesis is described by a differential am plifier with constant gain. Although thermokinesis heavily masks circadian responses to tem perature signals, the lim ited range o f circadian entrainment is indirectly dem onstrated by a lim ited reappearance o f free running circadian oscillations after stopping the tem perature program. Free running circadian oscillations do reappear only after pretreatment with tem perature periods near the circadian eigenperiod. A white mutant lacking photosynthesis is used to investigate the role o f photosynthesis in the signal processing. Although light synchronizes the circadian rhythms o f the white mutant if applied as single input, it does not affect the m otility if applied together with temperature inputs near the circadian eigenperiod. These results indicate frequency dependent m utual interactions between the model compartments. | | | |
Reference
| Z. Naturforsch. 39c, 801—8 (1984); received February 2 2 /April 27 1984 | | | |
Published
| 1984 | | | |
Keywords
| Circadian Rhythm, System Analysis, Euglena gracilis, Masking Temperature Responses | | | |
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| default:Reihe_C/39/ZNC-1984-39c-0801.pdf | | | | Identifier
| ZNC-1984-39c-0801 | | | | Volume
| 39 | |
| 7 | Author
| A. Kiinnea, E. Pistoriusb, K. Kloppstechc, E. De, G. Rootd | Requires cookie* | | | Title
| Circadian Synthesis of Light-Harvesting-Chlorophyll-Proteins in Euglena gracilis Is under Translational Control | | | | Abstract
| Two proteins with apparent molecular masses of 17 and 24 kD that are synthesized in a circadian manner in the phytoflagellate Euglena gracilis, were recognized as proteins belong ing to the family of light-harvesting-chlorophyll-proteins (LHCPs) of class 1(17 kD) and of class II (24 kD). Identification was achieved by N-terminal sequencing of the proteins iso lated from two-dimensional polyacrylamide gels and by detection with an anti-LHCP II se rum. While it was found that the total am ount of LHCPs remains almost constant, when Euglena is grown under diurnal conditions (12 h light and 12 h dark), we could show that the amount of newly synthesized 17 and 24 kD proteins varies about 20-fold with a maximum of synthesis in the light phase. In contrast, the analysis of the mRNA levels at different times revealed only minor differences in the stationary concentration of the LHCP specific mRNA, indicating that the control of LHCP synthesis is at the translational level. Principally, the same finding was obtained using inhibitors of transcription. Thus, it is concluded that the expression of LHCPs in Euglena gracilis in contrast to that of higher plants is primarily regulated at the translational level. | | | |
Reference
| Z. Naturforsch. 53c, 1017—1026 (1998); received July 7/August 3 1998 | | | |
Published
| 1998 | | | |
Keywords
| Euglena gracilis, Circadian Rhythm, Translational Control, Protein Synthesis, Light-Harvesting-Chlorophyll-Proteins | | | |
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| default:Reihe_C/53/ZNC-1998-53c-1017.pdf | | | | Identifier
| ZNC-1998-53c-1017 | | | | Volume
| 53 | |
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