| 1 | Author | Dieter Strack | Requires cookie* |
| Title | Enzymatic Synthesis of 1-Sinapoylglucose from Free Sinapic Acid and UDP-Glucose by a Cell-Free System from Raphanus sativus Seedlings  | ||
| Abstract | Protein extracts from seedlings of Raphanus sativus catalyze the transfer of the glucosyl moiety of UDP-glucose to the carboxyl group o f phenolic acids. Enzymatic activity was determined spec-trophotometrically by measuring the increase in absorbance at 360 nm and/or by the aid of high performance liquid chromatography (HPLC). From 12 phenolic acids tested as acceptors, sinapic acid was by far the best substrate. The glu-cosyltransfer to sinapic acid has a pH optimum near 7 and requires as SH group for activity, p-Chloromercuribenzoate (PCMB) inhibits activity, which can be restored by the addition of di-thiothreitol (DTT). The formation o f 1-sinapoylglucose was found to be a reversible reaction, sin ce the addition of UDP results in a breakdown o f the ester. | ||
| Reference | Z. Naturforsch. 35c, 204—2 (1980); received December 28 1979 | ||
| Published | 1980 | ||
| Keywords | Raphanus, Brassicaceae, Phenylpropanoid Metabolism, Glucose Ester, Esterification | ||
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