Go toArchive
Browse byFacets
Bookbag ( 0 )
'Esterification' in keywords Facet   Publication Year 1980  [X]
Results  1 Item
Sorted by   
Section
Publication Year
1980[X]
1Author    Dieter StrackRequires cookie*
 Title    Enzymatic Synthesis of 1-Sinapoylglucose from Free Sinapic Acid and UDP-Glucose by a Cell-Free System from Raphanus sativus Seedlings  
 Abstract    Protein extracts from seedlings of Raphanus sativus catalyze the transfer of the glucosyl moiety of UDP-glucose to the carboxyl group o f phenolic acids. Enzymatic activity was determined spec-trophotometrically by measuring the increase in absorbance at 360 nm and/or by the aid of high performance liquid chromatography (HPLC). From 12 phenolic acids tested as acceptors, sinapic acid was by far the best substrate. The glu-cosyltransfer to sinapic acid has a pH optimum near 7 and requires as SH group for activity, p-Chloromercuribenzoate (PCMB) inhibits activity, which can be restored by the addition of di-thiothreitol (DTT). The formation o f 1-sinapoylglucose was found to be a reversible reaction, sin­ ce the addition of UDP results in a breakdown o f the ester. 
  Reference    Z. Naturforsch. 35c, 204—2 (1980); received December 28 1979 
  Published    1980 
  Keywords    Raphanus, Brassicaceae, Phenylpropanoid Metabolism, Glucose Ester, Esterification 
  Similar Items    Find
 DEBUG INFO      
 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0204.pdf 
 Identifier    ZNC-1980-35c-0204 
 Volume    35