| | 1 | Author
| G. Erhild, N. Urm, Ann, D. Ieter Strack | Requires cookie* | | | Title
| Sinapine Esterase I. Characterization of Sinapine Esterase from Cotyledons of Raphanus sativus  | | | | Abstract
| From cotyledons o f Raphanus sativus (red radish) an esterase activity which catalyzes the hy drolysis o f sinapine into sinapic acid and choline has been isolated. The enzyme, which has a near absolute specificity, is not analogous with any esterase described in the literature. The reaction has a pH optim um o f 8.5 and the apparent K m is 1.95 x 10~5 m. The enzyme is relatively insensi tive to both physostigm ine (eserine) {K x = 1.73 x 10-4 m) and neostigm ine (A'i = 2 .1 3 x 10-4 m). Diisopropyl fluorophosphate (D F P) showed no inhibition and diethyl /?-nitrophenylphosphate (E 600) only a slight inhibitory effect at 10-5 m, respectively. Choline (10~2 m) was inhibitory but acetylcholine (1 0 -2 m) stimulated the enzyme activity. | | | |
Reference
| Z. Naturforsch. 34c, 715—720 (1979); received June 11 1979 | | | |
Published
| 1979 | | | |
Keywords
| Esterase, Sinapine, Sinapic Acid Esters, Raphanus, Brassicaceae, High-Performance Liquid Chro matography (HPLC) | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/34/ZNC-1979-34c-0715.pdf | | | | Identifier
| ZNC-1979-34c-0715 | | | | Volume
| 34 | |
|
