| 1 | Author | Y. Yamazaki, M.-R Kula | Requires cookie* |
| Title | Entwicklung neuer Plattentests zum Nachweis mikrobieller Hydrolysen von Estern und Oxidationen von 2-Hydroxycarbonsäuren Developm ent of New Plate Tests for the D etection of Microbial Hydrolysis of Esters and Oxidations of 2-HydroxycarboxyIic Acids  | ||
| Abstract | The application o f the thin-agar-layer coated filter culture technique (Z. Naturforsch. 42c, 1082 (1987)) has been extended to the detection of ester hydrolysis and 2-hydroxyacid oxidation by microbial colonies. The former was perform ed by spraying with brom ocresol purple and the latter with a salicylhydrazide reagent. Under the optim ized conditions, the hydrolysis activity of more than 20 [imol/h • g-wet cells and the oxidation activity o f more than 40 |j.mol/h • g-wet cells were usually detected directly on the filter-plate cultures o f bacteria, yeasts and molds. | ||
| Reference | Z. Naturforsch. 42c, 1187—1192 (1987); received July 30 1987 | ||
| Published | 1987 | ||
| Keywords | Esterase, M icrobial, O xidation, Indicator, Salicylhydrazide | ||
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