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'Escherichia coli' in keywords Facet   Publication Year 1982  [X]
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1982[X]
1Author    Akira TaketoRequires cookie*
 Title    Sensitivity of Escherichia coli to Viral Nucleic Acid. XVI. Temperature Conditions for Ca2+-Dependent DNA Uptake in Escherichia coli  
 Abstract    Properties of C a2+-or Ba2+-dependent transfection and transform ation in Escherichia coli were examined, using (PX174 replicative-form (R F) D NA and plasm id DNA. F or the transfection and transformation, a heat pulse step was dispensable and the yield o f transfectants was, in most E. coli strains, rather reduced by the heat treatment. The heat pulse step was also detrim ental for the transformation o f certain strains such as lipopolysaccharide mutants. The first stage o f the DNA uptake process (formation of DNA • recipient cell complex) was dependent on low tem perature and Ca2+ ion. A substantial am ount o f the complexed R F-D N A was released from the bacteria, by washing with a chilled Tris buffer. Although a R F-D N A • cell complex was formed even at 37 °C or in chilled 0.05 m MgCl2, the complex did not yield transfectants. 
  Reference    Z. Naturforsch. 37c, 87—92 (1982); received October 141981 
  Published    1982 
  Keywords    DNA, Transfection, Transformation, Escherichia coli 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0087.pdf 
 Identifier    ZNC-1982-37c-0087 
 Volume    37 
2Author    Arie Rosner, Marian Gorecki, Haim AvivRequires cookie*
 Title    Screening for Highly Active Plasmid Promoters via Fusion to /?-Galactosidase Gene  
 Abstract    A plasmid containing promoter-deleted inactive /?-galactosidase gene [1] was used to select promoters of the pEP121 plasmid [2]. Colonies o f cells harboring reactivated /?-galactosidase gene were identified by their red color on McConkey plates. The quantitative amounts o f /?-galactosidase produced in each clone were estimated by assaying enzyme activity and by measuring the specific /?-galactosidase protein following fractionation o f total cells' proteins on polyacrylamide gel. A wide range o f enzyme activities was observed. The most active promoter isolated was shown to promote /?-galactosidase production more efficiently, compared with the original /?-galactosidase promoter, amounting to 20% o f all cell proteins. Such highly active promoters may be utilized in the future, to promote expression o f cloned genes in bacteria. 
  Reference    Z. Naturforsch. 37c, 441—444 (1982); received January 13 1982 
  Published    1982 
  Keywords    Plasmid Promoter, /?-Galactosidase Gene, Escherichia coli 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0441.pdf 
 Identifier    ZNC-1982-37c-0441 
 Volume    37