ZfN: Search Results

1	Author	Akira Taketo	Requires cookie*
	Title	Ba2+-Induced Competence for Transfecting D NA
	Abstract	Effect of alkaline earth metal ions on induction of the competence for DNA transfection was investigated. Unlike spheroplasts, the bulk of the bacteria treated with these ions retains colony-forming ability. The order of effectiveness for transfection of replicative-form DNA has been found to be Ba2+> C a 2+> S r 2+> M g 2+. The competence of Ba2+-treated cells is 3 to 5 times higher than that of Ca^-treated bacteria and about 40 times higher than that of lysozyme-EDTA sphero plasts. The Ba^-dependent transfection is cryophilic and formation of the infective complex occurs very rapidly at 0 °C, but not at 37 °C.
	Reference	(Z. Naturforsch. 30c, 520—522 [1975]; received March 11 1975)
	Published	1975
	Keywords	Ba2+, Competence, DNA, Escherichia coli, Transfection
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	TEI-XML for	default:Reihe_C/30/ZNC-1975-30c-0520.pdf
	Identifier	ZNC-1975-30c-0520
	Volume	30

2	Author	Pavel Balgavý, Robert Turek	Requires cookie*
	Title	Inhibition of Excision Repair without Influence upon UV-Sensitivity and UV-Mutability in Escherichia coli B/r Hcr +
	Abstract	Pre-irradiation starvation of exponentially growing Escherichia coli B/r Hcr+ thy~trp~ strain for thymine and tryptophan causes inhibition of pyrimidine dimer excision from ultraviolet damaged cells DNA. This inhibition of excision repair has not resulted in increasing ultraviolet sensitivity nor in increasing frequency of ultraviolet induced tryptophan revertants. The possible mechanisms of the non-excision repair in the prestarved cells, which is at least as accurate and effective as the whole dark repair in exponentially growing cells, are discussed.
	Reference	(Z. Naturforsch. 30c, 406 [1975]; received August 1/November 22 1974)
	Published	1975
	Keywords	DNA-Repair, UV-Sensitivity, UV-Mutagenesis, Escherichia coli, UV-Radiation
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	TEI-XML for	default:Reihe_C/30/ZNC-1975-30c-0406.pdf
	Identifier	ZNC-1975-30c-0406
	Volume	30