| | 1 | Author
| Hans Gruler | Requires cookie* | | | Title
| Chemoelastic Effect of Membranes  | | | | Abstract
| The elastic theory of a uniaxial membrane in an asymmetric environment predicts a spontaneous splay deformation. This spontaneous curvature of the membrane is discussed by the intrinsic splay of the membrane molecules (e. g. wedge shaped molecules) and their polar orientation. The chemoelastic effect is the polar orientation induced by the asymmetric environment in connection with the intrinsic splay. This effect is also discussed for polyelectrolytes where a small change of pH (~0.1) can lead to a spontaneous curvature of 104 cm-1. The actual shape of red blood cells can be explained by the spontaneous splay and a change in environment induces the change in shape of these cells. A model is proposed for two conical bodies swimming in a uniaxial membrane which interact with each other through elastic coupling. The force between the bodies can be either attractive or repulsive. As an example of this model clustering of proteins is discussed. | | | |
Reference
| (Z. Naturforsch. 30c, 608—614 [1975]; received June 18 1975) | | | |
Published
| 1975 | | | |
Keywords
| Membrane, Elasticity, Erythrocyte, Enzyme Coupling | | | |
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| | | | | TEI-XML for
| default:Reihe_C/30/ZNC-1975-30c-0608.pdf | | | | Identifier
| ZNC-1975-30c-0608 | | | | Volume
| 30 | |
| 2 | Author
| Nicholas Zagris, CharlesG. Melton | Requires cookie* | | | Title
| Hemoglobins in Single Chick Erythrocytes as Determined by a Differential Elution Procedure | | | | Abstract
| The switch from embryonic to adult hemoglobin (Hb) has been studied in vivo by a correlated cytological and electrophoretic analysis of circulating red blood cells from early, purely embryonic-Hb stages to purely adult-Hb stages including the adult chicken. It has been discovered, by using an acid buffer treatment that selectively elutes adult but not embryonic Hb from intact red blood cells, that embryonic and adult Hbs occur together in single cells, and that the switch occurs simultaneously in all cells. These results together with knowledge of the chick erythroid cell dynamics and ontogenetic titers indicate that the initiation of adult Hb synthesis occurs in the circulation in cells previously committed only to embryonic Hb synthesis. | | | |
Reference
| Z. Naturforsch. 33c, 330 (1978); received January 17/March 10 1978 | | | |
Published
| 1978 | | | |
Keywords
| Chick, Erythrocyte, Hemoglobins, Selective Elution | | | |
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| | | | | TEI-XML for
| default:Reihe_C/33/ZNC-1978-33c-0330.pdf | | | | Identifier
| ZNC-1978-33c-0330 | | | | Volume
| 33 | |
| 3 | Author
| H. Alina, Kleszczyń Ska, Janusz Sarapuk | Requires cookie* | | | Title
| Influence of Organic and Inorganic Ions on Organolead-Induced Hemolysis of Erythrocytes | | | | Abstract
| The influence of some inorganic (K +, Mg2+, and A l3+) and organic C"H2"+1 S 0 3^, n -12 and 14) ions was studied on the hemolysis of erythrocytes (RBC) caused by organolead compounds (tripropyllead -TPL, tributyllead -TBL and triphenyllead -TPhL chlorides). It was found that sulfonate anions increased the hemolytic effect induced by triorganoleads, while inorganic cations protected RBC against the triorganoleads action, especially when the latter were used at small concentrations. This protection was weaker when the concentration of organoleads increased and depended on the kind of ion. The protective efficacy sequence was like that: Mg2+ > A l3+ > K +. The less hemolytic of the triorganoleads studied was TPL. TBL was slightly more effective than TPhL. The efficacy of the sulfonate ions to increase the triorganolead chloride -induced hemolysis was practically the same for TPL and TBL. A weaker efficacy of C i2H25S03+ was observed when TPhL was used as RBC membrane modifier. | | | |
Reference
| Z. Naturforsch. 56c, 853—856 (2001); received February 26/March 27 2001 | | | |
Published
| 2001 | | | |
Keywords
| Organoleads, Erythrocytes, Hemolysis Inhibition | | | |
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| | | | | TEI-XML for
| default:Reihe_C/56/ZNC-2001-56c-0853.pdf | | | | Identifier
| ZNC-2001-56c-0853 | | | | Volume
| 56 | |
| 4 | Author
| R. Hampp, C. Kriebitzsch | Requires cookie* | | | Title
| Effect of Zinc and Cadmium on 5-Aminolevulinate Dehydratase of Red Blood Cells in Protecting against Enzyme Losses during Storage | | | | Abstract
| The effect of zinc and cadmium on <5-aminolevulinate dehydratase of bovine erythrocytes stored at — 30 °C for different times was determined. The results show a. storage of erythrocytes leads to an enhancement of the enzyme activity, which after six weeks is 165% (500 [*.M ZnCl2) respectively 220% (100 /um CdCl2) for red blood cells of calves, and after four weeks is 420% respectively 450% (same concentrations) for red blood cells of adult cattle, b. the older the samples are, the higher is the metal concentration, needed for activation. | | | |
Reference
| (Z. Naturforsch. 30c, 434 [1975]; received March 5/April 151975) | | | |
Published
| 1975 | | | |
Keywords
| <5-Aminolevulinate Dehydratase, Erythrocytes, Zinc, Cadmium | | | |
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| | | | | TEI-XML for
| default:Reihe_C/30/ZNC-1975-30c-0434.pdf | | | | Identifier
| ZNC-1975-30c-0434 | | | | Volume
| 30 | |
| 7 | Author
| Peter Scheurich, Ulrich Zimmermann, Maja Mischel, Ingolf Lamprecht | Requires cookie* | | | Title
| Membrane Fusion and Deformation of Red Blood Cells by Electric Fields | | | | Abstract
| Human red blood cells suspended in a slightly hypotonic solution o f low electric conductivity were exposed to an inhomogeneous and alternating electric field (sine wave, 30 V peak-to-peak value, electrode distance 120 |im, 0.5 to 2 MHz). Due to the dielectrophoretic effect the cells align parallel to the field lines under the formation o f pearl chains. At high voltages (10 V amplitude) membrane fusion is observed between the adhered red blood cells in the pearl chains, whereby the chains become attached to the electrodes. In contrast to the pearl chains observed at voltages o f up to 5 V amplitude the resulting fused and uniform aggregates which exhibit no recognisable individual cells under the light microscope, remain stable, even after the alternating electric field has been switched off or after haemolysis in response to osmotic shock. The fused aggregates are highly elastic. If the field strength o f the applied alternating electric field is further increased they are stretched in the direction o f the opposite electrode. Frequently, bridges are formed between the two electrodes. The uniform bridges remain stable for some time even in the absence o f an electric field. The possibility o f cell fusion and its initiation by electrical breakdown of the cell membranes are discussed. | | | |
Reference
| Z. Naturforsch. 35c, 1081—1085 (1980); received August 21 1980 | | | |
Published
| 1980 | | | |
Keywords
| Deformability, Dielectrophoresis, Electric Breakdown, Erythrocytes, Fusion | | | |
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| default:Reihe_C/35/ZNC-1980-35c-1081.pdf | | | | Identifier
| ZNC-1980-35c-1081 | | | | Volume
| 35 | |
| 8 | Author
| T. K. Mandal, S. N. Chatterjee | Requires cookie* | | | Title
| Electron Microscopic Study of the Polymyxin Treated Goat Erythrocytes | | | | Abstract
| Polymyxin B produced dose dependent changes in the surface topography o f the goat erythrocyte cells. Transformation from the normal biconcave discs through crenated structures to the final rounded or spherical shape was recorded by scanning electron m icroscopy. A m axim um o f three to four crenations per cell was recorded corresponding to a polym yxin dose o f 15.62 ng/ml. Transmission electron m icroscopy o f the ultrathin sections o f treated or untreated erythrocytes indicated that the crenations were formed by protrusions o f the plasma membrane, occurring presumably because o f the local increase o f m em brane fluidity after polym yxin treatment. Changes in the shape o f the erythrocytes to the ultim ate rounded forms were also indicated by the transmission electron microscopy. | | | |
Reference
| Z. Naturforsch. 39c, 776—7 (1984); received April 24 1984 | | | |
Published
| 1984 | | | |
Keywords
| Polymyxin B, Erythrocytes, Surface Topography, Electron M icroscopy | | | |
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| default:Reihe_C/39/ZNC-1984-39c-0776.pdf | | | | Identifier
| ZNC-1984-39c-0776 | | | | Volume
| 39 | |
| 9 | Author
| C. Ballario, A. Bonincontro, C. Cametti, A. Rosi, L. Sportelli | Requires cookie* | | | Title
| Effect of Extracellular Alkali Metal Salts on the Electric Parameters of Human Erythrocytes in Normal and Pathological Conditions (Homozygous ^-Thalassemia) | | | | Abstract
| The conductivity of human erythrocyte cells dispersed in various uni-univalent electrolyte solutions (NaCl, KC1, LiCl, CsCl; 0.15 m) have been measured in the frequency range from 10 KHz to 100 MHz at five temperatures between 5 and 45 °C. The results were analyzed in the light of the theory of conductivity polarization of a suspension of ellipsoidal particles covered with two confocal shells. Differences in the electrical parameters of the membrane between normal and homozygous /?-thalassemic cells have been evidentiated. | | | |
Reference
| Z. Naturforsch. 39c, 1163—1169 (1984); received April 24/September 10 1984 | | | |
Published
| 1984 | | | |
Keywords
| Conductivity, Radiowave Frequencies, Electrolyte Solutions, Erythrocytes, Homozygous /?-Thalassemic Erythrocytes | | | |
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| | | | | TEI-XML for
| default:Reihe_C/39/ZNC-1984-39c-1163.pdf | | | | Identifier
| ZNC-1984-39c-1163 | | | | Volume
| 39 | |
| 10 | Author
| Arkadiusz Kozubek | Requires cookie* | | | Title
| Higher Cardol Homologues (5-Alkenylresorcinols) from Rye Affect the Red Cell Membrane-Water Transport | | | | Abstract
| The influence o f 5-heptadecenylresorcinol and total rye 5-alkenylresorcinols isolated from rye grains on the red blood cell water perm eability was studied using osm otic shrinkage experim ents performed in 300 m M sucrose. The studied com pounds induced significant increase o f erythro cyte water permeability. The threshold concentration needed for the increase o f water per meability was in an order o f 10_ 6 m ol/l. The temperature dependence o f the observed process showed the discontinuity which was related to the 5-alkenylresorcinol transition temperatures. It was shown also that alkenylresorcinols did not exert the biphasic action on hypotonic lysis o f erythrocytes usually observed for water soluble surfactants. The sp ecific lysine activity is postulated for the studied compounds. | | | |
Reference
| Z. Naturforsch. 40c, 80 (1985); received N ovem ber 2 1984 | | | |
Published
| 1985 | | | |
Keywords
| Phenolic Lipids, 5-Alkenylresorcinols, Rye, Erythrocytes, Water Perm eability | | | |
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| default:Reihe_C/40/ZNC-1985-40c-0080.pdf | | | | Identifier
| ZNC-1985-40c-0080 | | | | Volume
| 40 | |
| 11 | Author
| Fumio Yoshii, Isao Kaetsu, Akio Yamada | Requires cookie* | | | Title
| Immobilization of Erythrocytes by Radiation Polymerization of Glass-Forming Monomers at Low Temperatures | | | | Abstract
| The immobilization of erythrocyte as the whole cell without hemolysis was studied. It found that erythrocyte could be treated and immobilized stably by radiation polymerization o f specific monomers having high viscous and long oxyethylene units chain such as methoxypolyethylene-glycol methacrylate (M-23G) and polyethyleneglycol dimethacrylate (14G). Irradiation dose without hemolysis was limited less than lxl05r and a comonomer system consisting o f M -23G-14G, 1:1 and small quantity o f glutaraldehyde (GA) was the optimum carrier composition. The functional properties o f the immobilized erythrocyte was also investigated. It was found that the immobilized cell could be carried out carbon monoxide-oxygen gas exchange effectively and reversibly so as in the intact cell. The immobilized erythrocyte also showed the catalase activity just as in the intact cell. The stability o f erythrocyte increased greatly by the immobilization for standing at low and room temperatures and hardly hemolyzed in non-isotonic medium such as pure water. It was observed in scanning electron microscope that the immobilized erythrocyte had a hollow disk shape same as in intact cell and covered with a thin polymer layer. | | | |
Reference
| Z. Naturforsch. 36c, 1062—1067 (1981); received June 2 1981 | | | |
Published
| 1981 | | | |
Keywords
| Erythrocyte, Radiation Polymerization, Immobilization, Glass-Forming Monomer, Low Temperature, 0 2-Uptake | | | |
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| | | | | TEI-XML for
| default:Reihe_C/36/ZNC-1981-36c-1062.pdf | | | | Identifier
| ZNC-1981-36c-1062 | | | | Volume
| 36 | |
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