| | 1 | Author
| Fumio Yoshii, Isao Kaetsu, Akio Yamada | Requires cookie* | | | Title
| Immobilization of Erythrocytes by Radiation Polymerization of Glass-Forming Monomers at Low Temperatures  | | | | Abstract
| The immobilization of erythrocyte as the whole cell without hemolysis was studied. It found that erythrocyte could be treated and immobilized stably by radiation polymerization o f specific monomers having high viscous and long oxyethylene units chain such as methoxypolyethylene-glycol methacrylate (M-23G) and polyethyleneglycol dimethacrylate (14G). Irradiation dose without hemolysis was limited less than lxl05r and a comonomer system consisting o f M -23G-14G, 1:1 and small quantity o f glutaraldehyde (GA) was the optimum carrier composition. The functional properties o f the immobilized erythrocyte was also investigated. It was found that the immobilized cell could be carried out carbon monoxide-oxygen gas exchange effectively and reversibly so as in the intact cell. The immobilized erythrocyte also showed the catalase activity just as in the intact cell. The stability o f erythrocyte increased greatly by the immobilization for standing at low and room temperatures and hardly hemolyzed in non-isotonic medium such as pure water. It was observed in scanning electron microscope that the immobilized erythrocyte had a hollow disk shape same as in intact cell and covered with a thin polymer layer. | | | |
Reference
| Z. Naturforsch. 36c, 1062—1067 (1981); received June 2 1981 | | | |
Published
| 1981 | | | |
Keywords
| Erythrocyte, Radiation Polymerization, Immobilization, Glass-Forming Monomer, Low Temperature, 0 2-Uptake | | | |
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| default:Reihe_C/36/ZNC-1981-36c-1062.pdf | | | | Identifier
| ZNC-1981-36c-1062 | | | | Volume
| 36 | |
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