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'Erythrocyte Membrane' in keywords
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1Author    Halina Kleszczyńska3, Janusz Sarapuk3, MałgorzataO. Święcimskab, StanisławW. ItekbRequires cookie*
 Title    Antioxidative Activity of Some Quaternary Ammonium Salts Incorporated into Erythrocyte Membranes  
 Abstract    The antioxidative activity of two series of amphiphilic compounds from a group of qu ater­ nary ammonium salts has been investigated. They were so-called bifunctional surfactants synthesized to be used as common pesticides or as antioxidants. The latter application was to be ensured by providing the compounds studied with an antioxidant group. Studies on antioxidative possibilities of those compounds were performed on pig erythrocytes. D ue to their hydrophobic parts, they anchor in the erythrocyte membrane and influence the degree of lipid oxidation in the erythrocyte membrane subjected to UV radiation. It was found that compounds of both series decreased the oxidation of the membrane lipids. The inhibition of this oxidation increased with the length of their hydrophobic chains up to fourteen carbon atoms. The compounds of the longest hydrophobic chains showed a somewhat weaker antiox­ idative activity. O f the two series studied compounds were more effective having bromide ions as counterions. The corresponding compounds of a second series (chlorides) protected erythrocyte significantly weaker against oxidation. The effect of the compounds on fluidity of the erythrocyte membrane has been studied in order to explain the oxidation results. Change in fluidity of the erythrocyte ghost membranes was found also dependent on length of the hydrophobic part of the compounds and was more pronounced in the case of bro­ mide surfactants. The final conclusion is that the compounds studied can be succesfully used as antioxidant agents of good efficacy. 
  Reference    Z. Naturforsch. 55c, 976—980 (2000); received June 20/August 3 2000 
  Published    2000 
  Keywords    Erythrocyte Membrane, Antioxidant Activity, Fluidity 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0976.pdf 
 Identifier    ZNC-2000-55c-0976 
 Volume    55 
2Author    Mario Suwalsky3, Pedro Hernándeza, Fernando Villenab, CarlosP. SotomayorcRequires cookie*
 Title    The Anticancer Drug Chlorambucil Interacts with the Human Erythrocyte Membrane and Model Phospholipid Bilayers  
 Abstract    The plasma membrane has gained increasing attention as a possible target of antitumor drugs. It has been reported that they act as growth factor antagonists, growth factor receptor blockers, interfere with mitogenic signal transduction or exert direct cytotoxic effects. Chlor­ ambucil (4-[p-(bis[2-chloroethyl]amino)phenyl]butyric acid) is an alkylating agent widely used in the treatment of chronic lymphocytic leukaemia. Contradictory reports have been published concerning its interaction with cell membranes. Whereas a decrease in the fluidity of Ehrlich ascite tumor cells has been adduced, no evidences were found that chlorambucil changes membrane lipid fluidity and alkylating agents had effects in these systems even at highly toxic concentrations. Our results showed that chlorambucil at a dose equivalent to its therapeutical concentration in the plasma (3.6 (.im) caused the human erythrocyte membrane to develop cup-shaped forms (stomatocytes). Accordingly to the bilayer couple hypothesis, this means that the drug is inserted into the inner monolayer of the erythrocyte membrane, a conclusion supported by X-ray diffraction performed on multilayers of dimyristoylphospha-tidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the erythrocyte membrane, respectively. It is concluded that the cytotoxic effect of chlorambucil might be due to alter­ ation of the structure and therefore of the physiological properties of cell membranes such as fluidity, permeability, receptor and channel functions. 
  Reference    Z. Naturforsch. 54c, 1089—1095 (1999); received June 21/July 23 1999 
  Published    1999 
  Keywords    Chlorambucil, Anticancer Drug, Erythrocyte Membrane, Phospholipid Bilayer 
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 TEI-XML for    default:Reihe_C/54/ZNC-1999-54c-1089.pdf 
 Identifier    ZNC-1999-54c-1089 
 Volume    54 
3Author    R. Cicero3, D. Callarib, U. Id, N. Ic, V. V. Itic, M. Scaliad, I. M. Aid, A. Billitterib, G. SicheldRequires cookie*
 Title    Effects of Vitamin A in the Presence of Vitamins D3, E, K, on Red Cell Membrane Structure  
 Abstract    The incubation of erythrocyte ghosts with mixtures of vitamins A + D 3, A+E, A + K , produces decrease or increase of fluorescence anisotropy r of the DPH probe, depending on the vitamin/vitamin ratio. We found a correlation between the order parameter S and cell fusion phenomena observed by phase contrast microscopy. 
  Reference    Z. Naturforsch. 39c, 749—752 (1984); received January 3 1984 
  Published    1984 
  Keywords    Lipophilic Vitamins, Erythrocyte Membrane, Cell Fusion, Fluorescence Anisotropy 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0749.pdf 
 Identifier    ZNC-1984-39c-0749 
 Volume    39 
4Author    H. Kleszczyńska, J. Hładyszowski, H. Pruchnik, S. PrzestalskiRequires cookie*
 Title    Erythrocyte Hemolysis by Organic Tin and Lead Compounds  
 Abstract    The effect of trialkyllead and trialkyltin on pig erythrocyte hemolysis has been studied and compared. The results o f experiments showed that the hemolytic activity o f organoleads increases with their hydrophobicity and follows the sequence: triethyllead chloride < tri-n-propyllead chloride < tributyllead chloride. And similarly in the case of organotins: triethyltin chloride < tri-n-propyltin chloride < tributyltin chloride. Comparison of the hemolytic activity of organoleads and organotins indicates that the lead compounds exhibit higher hemolytic activity. The methods of quantum chemistry allowed to determine the maximum electric potential of the ions R3Pb+ and R3Sn+, and suggest a relationship betw een the potential and toxicity. 
  Reference    Z. Naturforsch. 52c, 65—6 (1997); received August 9/October 7 1996 
  Published    1997 
  Keywords    Organoleads, Organotins, Erythrocyte Membrane, Hemolysis, Electric Potential 
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 TEI-XML for    default:Reihe_C/52/ZNC-1997-52c-0065.pdf 
 Identifier    ZNC-1997-52c-0065 
 Volume    52 
5Author    Halina Kleszczyńska3, MałgorzataO. Święcimskab, Janusz Sarapuk3, Stanisław Witekb, Stanisław Przestalski3Requires cookie*
 Title    Protective Effect of Quaternary Piperidinium Salts on Lipid Oxidation in the Erythrocyte Membrane  
 Abstract    A new series of amphiphilic compounds with incorporated antioxidant functional group has been investigated. Piperidinium bromides, differing in the alkyl chain length (8, 10, 12, 14 and 16 carbon atoms in the chain) were synthesised to protect biological and/or model membranes against peroxidation and following negative consequences. Their antioxidant ac­ tivity was studied with erythrocytes subjected to UV radiation. The salts used inhibited lipid oxidation in the erythrocyte membrane. The degree of this inhibition depended on the alkyl chain length of the bromide used and increased with increasing alkyl chain length. A compar­ ison of the results obtained for piperidinium bromides with those obtained for the widely used antioxidant 3,5-di-r-butyl-4-hydroxytoluene (BH T) revealed that only two shortest alkyl chain salts were less efficient than BH T in protecting erythrocyte membranes. A similar comparison with antioxidant efficiency of flavonoids extracted from Rosa rugosa showed that they protected the membranes studied more weakly than the least effective eight-carbon alkyl chain piperidinium bromide. The three compounds of longest alkyl chains were the most active antioxidants. Their activities did not differ significantly. 
  Reference    Z. Naturforsch. 54c, 424 (1999); received January 22/February 24 1999 
  Published    1999 
  Keywords    Antioxidants, Lipid Oxidation, Erythrocyte Membrane, Quaternary Piperidinium Salts, Flavonoids 
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 TEI-XML for    default:Reihe_C/54/ZNC-1999-54c-0424.pdf 
 Identifier    ZNC-1999-54c-0424 
 Volume    54 
6Author    Sabine Lindenthalab, Uwe Scheuringa, H. Orst, R. Ufd, Zbigniew Kojroa, Winfried Haasea, Peter Petraschc, Dieter Schuberta, M. Ikrobiologie0, JohannW. Olfgang, G.Requires cookie*
 Title    Asymmetrie Reconstitution of the Erythrocyte Anion Transport System in Vesicles of Different Curvature: Implications for the Shape of the Band 3 Protein  
 Abstract    The anion transport protein o f the human erythrocyte membrane, band 3, was solubilized and purified in solutions o f the non-ionic detergent nonaethylene glycol lauryl ether and then reconstituted in spherical egg phosphatidylcholine bilayers as described earlier (U. Scheuring, K. Kollewe, W. Haase, and D. Schubert, J. Membrane Biol. 90, 1 2 3 -1 3 5 (1986)). The result­ ing paucilamellar proteoliposom es o f average diameter 70 nm were transformed into smaller vesicles by French press treatment and fractionated according to size by gel filtration. The smallest protein-containing liposom es obtained had diameters around 32 nm; still smaller vesi­ cles were free o f protein. All proteoliposom e samples studied showed a rapid sulfate efflux which was sensitive to specific inhibitors o f band 3-mediated anion exchange. In addition, the orientation o f the transport protein in the vesicle membranes was found to be "right-side-out" in all samples. This suggests that the orientation o f the protein in the vesicle membranes is dictated by the shape o f the protein's intramembrane domain and that this domain has the form o f a truncated cone or pyramid. 
  Reference    Z. Naturforsch. 45c, 1021 (1990); received July 18/August 24 1990 
  Published    1990 
  Keywords    Band 3 Protein, A nion Transport System, Asymmetrie Reconstitution, Protein Shape, Erythrocyte Membrane 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-1021.pdf 
 Identifier    ZNC-1990-45c-1021 
 Volume    45 
7Author    H. Kleszczyńska3, J. Ł. Uczyńskib, S. Witekb, S. PrzestalskiaRequires cookie*
 Title    Hemolytic Activity of Aminoethyl-dodecanoates  
 Abstract    The effect of new lysosomotropic compounds on red blood cell hemolysis and erythrocyte membrane fluidity has been investigated. In earlier studies it was shown that the compounds inhibit the growth of yeast and plasma membrane H+-ATPase activity. The study was per­ formed with eight aminoethyl esters of lauric acid variously substituted at nitrogen atom. Esters of dodecanoic acid were chosen for study because at that chain length dimethylamino-ethyl esters showed maximum activity. The hemolytic activity of the substances studied exhib­ its diversified activity in their interaction with the erythrocyte membrane: they differ in hemolytic activity and affect membrane fluidity differently. Erythrocyte membrane fluidity changes under the effect of those compounds which possess highest hemolytic activity. The hemolytic activity of the aminoesters investigated was found to follow a sequence that de­ pended on basicity (i.e. ability of the protonated form formation) of the compound and its polar head group size. The most active are the compounds that possess not more than four carbon atoms substituted at nitrogen and highest pKa value. 
  Reference    Z. Naturforsch. 53c, 101 (1998); received November 3/December 1 1997 
  Published    1998 
  Keywords    Lysosomotropic Substances, Aminoesters of Fatty Acids, Erythrocyte Membrane, Hemolytic Activity, Fluidity 
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 TEI-XML for    default:Reihe_C/53/ZNC-1998-53c-0101.pdf 
 Identifier    ZNC-1998-53c-0101 
 Volume    53