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'Erythrocyte Membrane' in keywords Facet   Publication Year 1999  [X]
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1Author    Mario Suwalsky3, Pedro Hernándeza, Fernando Villenab, CarlosP. SotomayorcRequires cookie*
 Title    The Anticancer Drug Chlorambucil Interacts with the Human Erythrocyte Membrane and Model Phospholipid Bilayers  
 Abstract    The plasma membrane has gained increasing attention as a possible target of antitumor drugs. It has been reported that they act as growth factor antagonists, growth factor receptor blockers, interfere with mitogenic signal transduction or exert direct cytotoxic effects. Chlor­ ambucil (4-[p-(bis[2-chloroethyl]amino)phenyl]butyric acid) is an alkylating agent widely used in the treatment of chronic lymphocytic leukaemia. Contradictory reports have been published concerning its interaction with cell membranes. Whereas a decrease in the fluidity of Ehrlich ascite tumor cells has been adduced, no evidences were found that chlorambucil changes membrane lipid fluidity and alkylating agents had effects in these systems even at highly toxic concentrations. Our results showed that chlorambucil at a dose equivalent to its therapeutical concentration in the plasma (3.6 (.im) caused the human erythrocyte membrane to develop cup-shaped forms (stomatocytes). Accordingly to the bilayer couple hypothesis, this means that the drug is inserted into the inner monolayer of the erythrocyte membrane, a conclusion supported by X-ray diffraction performed on multilayers of dimyristoylphospha-tidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the erythrocyte membrane, respectively. It is concluded that the cytotoxic effect of chlorambucil might be due to alter­ ation of the structure and therefore of the physiological properties of cell membranes such as fluidity, permeability, receptor and channel functions. 
  Reference    Z. Naturforsch. 54c, 1089—1095 (1999); received June 21/July 23 1999 
  Published    1999 
  Keywords    Chlorambucil, Anticancer Drug, Erythrocyte Membrane, Phospholipid Bilayer 
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 TEI-XML for    default:Reihe_C/54/ZNC-1999-54c-1089.pdf 
 Identifier    ZNC-1999-54c-1089 
 Volume    54 
2Author    Halina Kleszczyńska3, MałgorzataO. Święcimskab, Janusz Sarapuk3, Stanisław Witekb, Stanisław Przestalski3Requires cookie*
 Title    Protective Effect of Quaternary Piperidinium Salts on Lipid Oxidation in the Erythrocyte Membrane  
 Abstract    A new series of amphiphilic compounds with incorporated antioxidant functional group has been investigated. Piperidinium bromides, differing in the alkyl chain length (8, 10, 12, 14 and 16 carbon atoms in the chain) were synthesised to protect biological and/or model membranes against peroxidation and following negative consequences. Their antioxidant ac­ tivity was studied with erythrocytes subjected to UV radiation. The salts used inhibited lipid oxidation in the erythrocyte membrane. The degree of this inhibition depended on the alkyl chain length of the bromide used and increased with increasing alkyl chain length. A compar­ ison of the results obtained for piperidinium bromides with those obtained for the widely used antioxidant 3,5-di-r-butyl-4-hydroxytoluene (BH T) revealed that only two shortest alkyl chain salts were less efficient than BH T in protecting erythrocyte membranes. A similar comparison with antioxidant efficiency of flavonoids extracted from Rosa rugosa showed that they protected the membranes studied more weakly than the least effective eight-carbon alkyl chain piperidinium bromide. The three compounds of longest alkyl chains were the most active antioxidants. Their activities did not differ significantly. 
  Reference    Z. Naturforsch. 54c, 424 (1999); received January 22/February 24 1999 
  Published    1999 
  Keywords    Antioxidants, Lipid Oxidation, Erythrocyte Membrane, Quaternary Piperidinium Salts, Flavonoids 
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 TEI-XML for    default:Reihe_C/54/ZNC-1999-54c-0424.pdf 
 Identifier    ZNC-1999-54c-0424 
 Volume    54