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'Erythrocyte Membrane' in keywords Facet   Publication Year 1990  [X]
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1990[X]
1Author    Sabine Lindenthalab, Uwe Scheuringa, H. Orst, R. Ufd, Zbigniew Kojroa, Winfried Haasea, Peter Petraschc, Dieter Schuberta, M. Ikrobiologie0, JohannW. Olfgang, G.Requires cookie*
 Title    Asymmetrie Reconstitution of the Erythrocyte Anion Transport System in Vesicles of Different Curvature: Implications for the Shape of the Band 3 Protein  
 Abstract    The anion transport protein o f the human erythrocyte membrane, band 3, was solubilized and purified in solutions o f the non-ionic detergent nonaethylene glycol lauryl ether and then reconstituted in spherical egg phosphatidylcholine bilayers as described earlier (U. Scheuring, K. Kollewe, W. Haase, and D. Schubert, J. Membrane Biol. 90, 1 2 3 -1 3 5 (1986)). The result­ ing paucilamellar proteoliposom es o f average diameter 70 nm were transformed into smaller vesicles by French press treatment and fractionated according to size by gel filtration. The smallest protein-containing liposom es obtained had diameters around 32 nm; still smaller vesi­ cles were free o f protein. All proteoliposom e samples studied showed a rapid sulfate efflux which was sensitive to specific inhibitors o f band 3-mediated anion exchange. In addition, the orientation o f the transport protein in the vesicle membranes was found to be "right-side-out" in all samples. This suggests that the orientation o f the protein in the vesicle membranes is dictated by the shape o f the protein's intramembrane domain and that this domain has the form o f a truncated cone or pyramid. 
  Reference    Z. Naturforsch. 45c, 1021 (1990); received July 18/August 24 1990 
  Published    1990 
  Keywords    Band 3 Protein, A nion Transport System, Asymmetrie Reconstitution, Protein Shape, Erythrocyte Membrane 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-1021.pdf 
 Identifier    ZNC-1990-45c-1021 
 Volume    45