| | 1 | Author
| Joseph Veser, P. Eter, G. | Requires cookie* | | | Title
| Purification and Properties of a Catechol Methyltransferase of the Yeast Candida tropicalis  | | | | Abstract
| In an effort to investigate catechol methyltransferase activity in sources other than mam m alian tissues and cells, a high level o f enzyme activity was found in the yeast fungus Candida tropicalis CBS 94. Partial purification o f the enzyme (approx. 550 fold with a recovery o f 7%) could be achieved by using ion-exchange and gel filtration techniques. The molecular w eight was estimated at 32,000 ± 2,000 by gel filtration on Sephadex G-100. In isoelectric focusing experiments on Sephadex G-75 the enzym e exhibited a pl-value o f 5.0 ± 0.1. In contrast to catechol methyltransfer ase from various m am m alian tissues the enzyme activity was prepared from the pH 5-sediment. The substrate specifity is com parable to other catechol methyltransferases. | | | |
Reference
| Z. Naturforsch. 34c, 709 (1979); received April 24 1979 | | | |
Published
| 1979 | | | |
Keywords
| Catechol Methyltransferase, Yeast Fungus, Candida tropicalis CBS 94, Enzyme Purification, Catechols | | | |
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| default:Reihe_C/34/ZNC-1979-34c-0709.pdf | | | | Identifier
| ZNC-1979-34c-0709 | | | | Volume
| 34 | |
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