| | 1 | Author
| PaavoH. Hynninen3, Riikka Räisänen3, Pia Elovaarab, Eila Nokelainenb | Requires cookie* | | | Title
| Preparative Isolation of Anthraquinones from the Fungus Dermocybe sanguined Using Enzymatic Hydrolysis by the Endogenous ß-Glucosidase  | | | | Abstract
| A new and simple enzymatic method was developed for preparative isolation of anthraqui none pigments from Dermocybe sanguined. The endogenous ß-glucosidase of the fungus was used to catalyze the hydrolysis of the O-glycosyl linkage in emodin-and dermocybin-1-ß-D-glucopyranosides. The developed enzymatic method was found to be effective for the pig ment isolation, as the hydrolysis occurred virtually completely, thus leading to a high pigment yield. Two fractions were obtained by the method: Fraction 1 (94% of the total pigment amount), containing almost exclusively the main pigments emodin and dermocybin, and Frac tion 2 (6 %), containing the anthraquinone carboxylic acids. A 10.5 kg amount of fresh fungi yielded 56 g of Fraction 1 and 3.3 g of Fraction 2 anthraquinones. The anthraquinones in each fraction were separated by thin-layer chromatography using tolu en e-eth yl acetate-eth anol-form ic acid (10:8:1:2, v/v/v/v) as eluent. The components on the chromatograms were detected and characterized by measurements on a densitom eter-spectrophotom eter. Combined gas chromatography-mass spectrom etry was applied to determine the anthraqui none derivatives of Fraction 1 after methylation and acetylation. | | | |
Reference
| Z. Naturforsch. 55c, 600 (2000); received March 22/April 25 2000 | | | |
Published
| 2000 | | | |
Keywords
| ß-Glucosidase, Enzymatic Hydrolysis, Natural Anthraquinone | | | |
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| default:Reihe_C/55/ZNC-2000-55c-0600.pdf | | | | Identifier
| ZNC-2000-55c-0600 | | | | Volume
| 55 | |
| 2 | Author
| Minoru Kumakura, Isao Kaetsu | Requires cookie* | | | Title
| Effect of Organic Solvents on Enzymatic Hydrolysis of Celluloses | | | | Abstract
| Effect of organic solvents on enzymatic hydrolysis of celluloses was studied. It was found that toluene and xylene can accelerate the rate of the enzymatic hydrolysis reaction of filter paper and cellulose powder. The addition o f N,N-dimethylformamide, acetone, and alcohols was negative effect for the enzymatic hydrolysis o f solid celluloses. In the addition o f alcohols and glycols, the relative glucose yields increased with increasing the carbon number o f their solvents. The addition o f organic solvents on the enzymatic hydrolysis of celluloses was discussed with swelling effect. It was found that the glucose yield increased as the dipole moment o f the solvents decreased. | | | |
Reference
| Z. Naturforsch. 38c, 79 (1983); received June 8/August 23 1982 | | | |
Published
| 1983 | | | |
Keywords
| Organic solvent, Enzymatic Hydrolysis, Cellulose, Acceleration Effect, Glucose Yield | | | |
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| default:Reihe_C/38/ZNC-1983-38c-0079.pdf | | | | Identifier
| ZNC-1983-38c-0079 | | | | Volume
| 38 | |
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