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'Enzymatic Hydrolysis' in keywords Facet   Publication Year 2000  [X]
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1Author    PaavoH. Hynninen3, Riikka Räisänen3, Pia Elovaarab, Eila NokelainenbRequires cookie*
 Title    Preparative Isolation of Anthraquinones from the Fungus Dermocybe sanguined Using Enzymatic Hydrolysis by the Endogenous ß-Glucosidase  
 Abstract    A new and simple enzymatic method was developed for preparative isolation of anthraqui­ none pigments from Dermocybe sanguined. The endogenous ß-glucosidase of the fungus was used to catalyze the hydrolysis of the O-glycosyl linkage in emodin-and dermocybin-1-ß-D-glucopyranosides. The developed enzymatic method was found to be effective for the pig­ ment isolation, as the hydrolysis occurred virtually completely, thus leading to a high pigment yield. Two fractions were obtained by the method: Fraction 1 (94% of the total pigment amount), containing almost exclusively the main pigments emodin and dermocybin, and Frac­ tion 2 (6 %), containing the anthraquinone carboxylic acids. A 10.5 kg amount of fresh fungi yielded 56 g of Fraction 1 and 3.3 g of Fraction 2 anthraquinones. The anthraquinones in each fraction were separated by thin-layer chromatography using tolu en e-eth yl acetate-eth anol-form ic acid (10:8:1:2, v/v/v/v) as eluent. The components on the chromatograms were detected and characterized by measurements on a densitom eter-spectrophotom eter. Combined gas chromatography-mass spectrom etry was applied to determine the anthraqui­ none derivatives of Fraction 1 after methylation and acetylation. 
  Reference    Z. Naturforsch. 55c, 600 (2000); received March 22/April 25 2000 
  Published    2000 
  Keywords    ß-Glucosidase, Enzymatic Hydrolysis, Natural Anthraquinone 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0600.pdf 
 Identifier    ZNC-2000-55c-0600 
 Volume    55