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2000 (1)
1983 (1)
1Author    PaavoH. Hynninen3, Riikka Räisänen3, Pia Elovaarab, Eila NokelainenbRequires cookie*
 Title    Preparative Isolation of Anthraquinones from the Fungus Dermocybe sanguined Using Enzymatic Hydrolysis by the Endogenous ß-Glucosidase  
 Abstract    A new and simple enzymatic method was developed for preparative isolation of anthraqui­ none pigments from Dermocybe sanguined. The endogenous ß-glucosidase of the fungus was used to catalyze the hydrolysis of the O-glycosyl linkage in emodin-and dermocybin-1-ß-D-glucopyranosides. The developed enzymatic method was found to be effective for the pig­ ment isolation, as the hydrolysis occurred virtually completely, thus leading to a high pigment yield. Two fractions were obtained by the method: Fraction 1 (94% of the total pigment amount), containing almost exclusively the main pigments emodin and dermocybin, and Frac­ tion 2 (6 %), containing the anthraquinone carboxylic acids. A 10.5 kg amount of fresh fungi yielded 56 g of Fraction 1 and 3.3 g of Fraction 2 anthraquinones. The anthraquinones in each fraction were separated by thin-layer chromatography using tolu en e-eth yl acetate-eth anol-form ic acid (10:8:1:2, v/v/v/v) as eluent. The components on the chromatograms were detected and characterized by measurements on a densitom eter-spectrophotom eter. Combined gas chromatography-mass spectrom etry was applied to determine the anthraqui­ none derivatives of Fraction 1 after methylation and acetylation. 
  Reference    Z. Naturforsch. 55c, 600 (2000); received March 22/April 25 2000 
  Published    2000 
  Keywords    ß-Glucosidase, Enzymatic Hydrolysis, Natural Anthraquinone 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0600.pdf 
 Identifier    ZNC-2000-55c-0600 
 Volume    55 
2Author    Minoru Kumakura, Isao KaetsuRequires cookie*
 Title    Effect of Organic Solvents on Enzymatic Hydrolysis of Celluloses  
 Abstract    Effect of organic solvents on enzymatic hydrolysis of celluloses was studied. It was found that toluene and xylene can accelerate the rate of the enzymatic hydrolysis reaction of filter paper and cellulose powder. The addition o f N,N-dimethylformamide, acetone, and alcohols was negative effect for the enzymatic hydrolysis o f solid celluloses. In the addition o f alcohols and glycols, the relative glucose yields increased with increasing the carbon number o f their solvents. The addition o f organic solvents on the enzymatic hydrolysis of celluloses was discussed with swelling effect. It was found that the glucose yield increased as the dipole moment o f the solvents decreased. 
  Reference    Z. Naturforsch. 38c, 79 (1983); received June 8/August 23 1982 
  Published    1983 
  Keywords    Organic solvent, Enzymatic Hydrolysis, Cellulose, Acceleration Effect, Glucose Yield 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0079.pdf 
 Identifier    ZNC-1983-38c-0079 
 Volume    38