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1993 (1)
1Author    SeungR. Yong, D. Ieter, W. G. Ruenw EdelRequires cookie*
 Title    Differential Effect of Hg(II) on [d(A)n * d(T)n] and [d(A-T)n * d(A-T)n] Sequences: Circular Dichroism (CD) Measurements and Endonuclease Digestion Studies Using Poly|d(A) * d(T)] and Poly[d(A-T) * d(A-T)] as Substrates  
 Abstract    The long-wavelength positive CD bands o f poly[d(A) • d(T)] and poly[d(A-T)-d(A-T)] be­ come inverted upon the addition of Hg(C104)2. Poly[d(A)-d(T)] requires higher levels of mer­ cury to undergo inversion than poly[d(A-T)-d(A-T)]\ Mercurated poly[d(A) d(T)] is digested more rapidly than the control by DNase I or staphylococcal nuclease at low levels of Hg(C104)2. Let r = [Hg(C104)2]added/[DNA-P]. A 4-to 5-fold rate increase occurs with DNase I at r = 0.25; a 2-fold increase with staphylococcal nuclease at r = 0.2. By contrast, digestion of poly[d(A-T) d(A-T)] decreases immediately with increasing r. The noted rate increases appear to be due to a modification of poly[d(A)-d(T)] helix structure prior to the chiroptical conver­ sion. The modification is interpreted as a widening of the minor groove, permitting, thus, a better binding of DNase I to its substrate. The overall changes in CD as well as enzymatic digestion rates are taken to signal mercury-induced alterations in helix screwness from right-to-left. They are totally reversible subsequent to the removal o f mercury. 
  Reference    Z. Naturforsch. 48c, 488—494 (1993); received September 18 1992/February 2 1993 
  Published    1993 
  Keywords    Circular Dichroism, Endonucleases, Polynucleotides, Mercury Complexes, Right«-*Left Inversions 
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 TEI-XML for    default:Reihe_C/48/ZNC-1993-48c-0488.pdf 
 Identifier    ZNC-1993-48c-0488 
 Volume    48