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1Author    A. Kio, K. Obayashi, K. Ohki, A. Kiyam, K. Azuyoshi, K. Aw AzuRequires cookie*
 Title    Partially N-Deacetylated Chitin Fragments are Strong Elicitors for (+)-Pisatin Induction in Epicotyls of Pea  
 Abstract    Chitosan derivatives classed into three groups according to their molecular size were exam­ ined for the relationships between degree of N -acetylation and phytoalexin (+)-pisatin-induc-ing activity in pea epicotyl assay. Partially N-deacetylated chitin with degree o f N-acetylation 56% (DAC 56%) was the most potent inducer o f (+)-pisatin am ong sparingly water-soluble polysaccharides including chitosan (degree o f N -acetylation 0%), DAC 32%, and chitin. Among the intermediate-sized molecules obtained from degradation of the chitosan and the DACs by N a N 0 2, the chitosan fragment was the most powerful elicitor for (+)-pisatin induc­ tion. The N a N 0 2-degraded DAC 56% exhibited a com parable activity to the N a N 0 2 -degrad-ed chitosan at low concentrations. The N a N 0 2-degraded fragments as well as the native poly­ mer induced a relatively high am ount of (+)-pisatin, while the acetone precipitate of chitosan oligomers from the HCl-catalyzed hydrolysate and their partially N-acetylated derivatives ex­ hibited a m oderate (+)-pisatin-inducing activity. These findings suggest the possibility that partially N-deacetylated chitin fragments could be one o f the most promising elicitors in the pea system. 
  Reference    Z. Naturforsch. 49c, 302—308 (1994); received January 14/February 24 1994 
  Published    1994 
  Keywords    Elicitor, Chitosan, Chitin Pea, Phytoalexin 
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 TEI-XML for    default:Reihe_C/49/ZNC-1994-49c-0302.pdf 
 Identifier    ZNC-1994-49c-0302 
 Volume    49