| | 1 | Author
| Joachim Bollmann, Klaus Hahlbrock | Requires cookie* | | | Title
| Timing of Changes in Protein Synthesis Pattern in Elicitor-Treated Cell Suspension Cultures of Parsley ( Petroselinum crispum)  | | | | Abstract
| The timing o f changes in the protein synthesis pattern o f elicitor-treated, [35S]methionine-labelled parsley cells (Petroselinum crispum) was analyzed by two-dimensional gel electropho resis. Five groups were distinguished from a large number o f elicitor-responsive as well as un responsive proteins. Two groups were synthesized de novo either early or late after elicitor ap plication; two other groups were strongly reduced in their rates o f synthesis either early or late after elicitor application; and one group was not appreciably affected at all. The elicitor-in-duced changes altered the total protein com position considerably. A few selected, induced pro teins were functionally identified. These included two early induced enzymes, phenylalanine amm onia-lyase (PAL) and 4-coum arate:C oA ligase (4C L), and a late induced enzyme, a ber-gaptol O-methyltransferase (BM T) which is specifically involved in the biosynthesis o f furano-coumarin phytoalexins. The biological significance o f the observed differential timing o f changes in protein synthesis rates is discussed. | | | |
Reference
| Z. Naturforsch. 45c, 1011 (1990); received May 30 1990 | | | |
Published
| 1990 | | | |
Keywords
| Cell Culture, Elicitor, Petroselinum crispum, Protein Synthesis, Two-Dim ensional Gel Electrophoresis | | | |
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| default:Reihe_C/45/ZNC-1990-45c-1011.pdf | | | | Identifier
| ZNC-1990-45c-1011 | | | | Volume
| 45 | |
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