| | 1 | Author
| H. O. Sk Ar, Schm Idt, K. Arl-, H. Einz Süss | Requires cookie* | | | Title
| Separation of Chloroplast Thylakoid Membrane Polypeptides by Electrophoresis on Polyacrylamide Gradient Gels with a Length of 60 cm  | | | | Abstract
| 14C labeled thylakoid membranes of Viciafaba chloroplasts were separated by one-dimensional electrophoresis in gel cuvettes of different length using a 12 — 18% polyacrylamide gradient gel system containing 0.1% sodiumodecylsulfate and 6 m urea. The use of gels with a length of 22 cm and 60 cm led to the detection of about 40 and 80 polypeptide bands, respectively, after dye staining and 50 and more than 100 polypeptide bands, respectively, after autoradiography. The conditions used strongly denaturate chlorophyll proteins and avoid the formation of different polypeptide conformations. Polypeptides differing in were separated in sharp bands. | | | |
Reference
| Z. Naturforsch. 40c, 705—709 (1985); received May 29 1985 | | | |
Published
| 1985 | | | |
Keywords
| Chloroplast, Thylakoid Membrane Proteins, Electrophoresis, Polyacrylamide, Coomassie Blue Stain | | | |
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| default:Reihe_C/40/ZNC-1985-40c-0705.pdf | | | | Identifier
| ZNC-1985-40c-0705 | | | | Volume
| 40 | |
| 3 | Author
| Hans-Joachim Bode | Requires cookie* | | | Title
| A Viscosity Model of Polyacrylamide Gel Electrophoresis | | | | Abstract
| In current theories o f polyacrylam ide gel electrophoresis, the idea prevails that molecular siev ing relies on different accessibility o f volum e fractions and o f cross-sectional area fractions (denot ed "pores") to different-sized ions due to the effect o f "geom etric exclusion". This correlates with the assum ption that all elements o f a polyacrylam ide network occupy fixed and unchangeable po sitions thus forcing colliding macro-ions to diffuse laterally in order to find an "accessible pore" and to resume m otion in direction o f the electrical field. H owever, the alternative conception would be equally well justified, i. e. the assum ption that polyacrylam ide chains represent smooth obstacles cleared aside under the electrokinetic pressure o f a macro-ion. This explanation would even be preferable with respect to the m olecular sieving effects occuring in solutions o f "liquid polyacrylam ide". Yet no theory exists as to describe such effects in quantitative terms. In the present article, a parameter is defined and discussed, w hich can be estim ated by experi ment, and which seems to be apt to characterize local resistivity o f polymer structures against dis location and deformation: the "fractional specific resistance" . D efinition o f this parameter is bas ed on the m odel o f a "viscosity-em ulsion" com posed o f two interpenetrating liquid compartments which are characterized by different levels o f hydrodynam ic friction and the spatial dimensions o f which are inferred from Ogston's theory. This concept o f "localized viscosity" m ay also serve as a link between theories o f molecular sieving and o f "m acroscopic viscosity" o f flexible polymers. The data o f Morris, formerly taken as verifications o f the "rigid-pore" concept, are now interpret ed in terms o f four factors responsible for sizediscrimination: collision frequency, duration o f sin gle contacts, size-dependent frictional force, and the extent o f cooperation am ong fibres, due to crosslinking and to simultanous contacts o f several fibres to a single macro-ion. Som e functions rel evant for problem s o f molecular weight determ ination by gel electrophoresis are discussed in rela tion to the suggested model. | | | |
Reference
| Z. Naturforsch. 34c, 512—528 (1979); received March 22 1979 | | | |
Published
| 1979 | | | |
Keywords
| Polyacrylamide, M olecular Sieve, Electrophoresis, Viscosity, Frictional Coefficient, Partition C oefficient | | | |
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| default:Reihe_C/34/ZNC-1979-34c-0512.pdf | | | | Identifier
| ZNC-1979-34c-0512 | | | | Volume
| 34 | |
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