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1998 (1)
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1Author    H. O. Sk Ar, Schm Idt, K. Arl-, H. Einz SüssRequires cookie*
 Title    Separation of Chloroplast Thylakoid Membrane Polypeptides by Electrophoresis on Polyacrylamide Gradient Gels with a Length of 60 cm  
 Abstract    14C labeled thylakoid membranes of Viciafaba chloroplasts were separated by one-dimensional electrophoresis in gel cuvettes of different length using a 12 — 18% polyacrylamide gradient gel system containing 0.1% sodiumodecylsulfate and 6 m urea. The use of gels with a length of 22 cm and 60 cm led to the detection of about 40 and 80 polypeptide bands, respectively, after dye staining and 50 and more than 100 polypeptide bands, respectively, after autoradiography. The conditions used strongly denaturate chlorophyll proteins and avoid the formation of different polypeptide conformations. Polypeptides differing in were separated in sharp bands. 
  Reference    Z. Naturforsch. 40c, 705—709 (1985); received May 29 1985 
  Published    1985 
  Keywords    Chloroplast, Thylakoid Membrane Proteins, Electrophoresis, Polyacrylamide, Coomassie Blue Stain 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0705.pdf 
 Identifier    ZNC-1985-40c-0705 
 Volume    40 
2Author    Julia Yanevaa, Jordanka Zlatanova30Requires cookie*
 Title    The Electrophoretic Separation of Curved Cisplatin-Modifled DNA Fragments on Polyacrylamide Gels Is Dependent on the Voltage Gradient  
 Abstract    Polyacrylamide gel electrophoresis has been widely used to study DNA fragments containing sequence-de-pendent curvature. The anomalous electrophoretic be­ havior of curved DNA fragments on such gels allows their separation from straight fragments of the same length. H ere we dem onstrate that polyacrylamide gels can be successfully used to resolve DNA fragments m od­ ified at a single site by the antitum or drug ds-diam-m inedichloroplatinum (II) (ds-DDP, cisplatin) from their unmodified counterparts. However, the resolution strongly depends on the voltage gradient, being com­ pletely lost when it drops below a certain threshold level. The param eters of the electric field do not affect separation of 'norm al' DNA fragments of comparable length. 
  Reference    Z. Naturforsch. 53c, 921—923 (1998); received April 27/May 12 1998 
  Published    1998 
  Keywords    Cisplatin, Curved DNA, Electric Field, Electrophoresis, Polyacrylamide Gels 
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 TEI-XML for    default:Reihe_C/53/ZNC-1998-53c-0921_n.pdf 
 Identifier    ZNC-1998-53c-0921_n 
 Volume    53 
3Author    Hans-Joachim BodeRequires cookie*
 Title    A Viscosity Model of Polyacrylamide Gel Electrophoresis  
 Abstract    In current theories o f polyacrylam ide gel electrophoresis, the idea prevails that molecular siev­ ing relies on different accessibility o f volum e fractions and o f cross-sectional area fractions (denot­ ed "pores") to different-sized ions due to the effect o f "geom etric exclusion". This correlates with the assum ption that all elements o f a polyacrylam ide network occupy fixed and unchangeable po­ sitions thus forcing colliding macro-ions to diffuse laterally in order to find an "accessible pore" and to resume m otion in direction o f the electrical field. H owever, the alternative conception would be equally well justified, i. e. the assum ption that polyacrylam ide chains represent smooth obstacles cleared aside under the electrokinetic pressure o f a macro-ion. This explanation would even be preferable with respect to the m olecular sieving effects occuring in solutions o f "liquid polyacrylam ide". Yet no theory exists as to describe such effects in quantitative terms. In the present article, a parameter is defined and discussed, w hich can be estim ated by experi­ ment, and which seems to be apt to characterize local resistivity o f polymer structures against dis­ location and deformation: the "fractional specific resistance" . D efinition o f this parameter is bas­ ed on the m odel o f a "viscosity-em ulsion" com posed o f two interpenetrating liquid compartments which are characterized by different levels o f hydrodynam ic friction and the spatial dimensions o f which are inferred from Ogston's theory. This concept o f "localized viscosity" m ay also serve as a link between theories o f molecular sieving and o f "m acroscopic viscosity" o f flexible polymers. The data o f Morris, formerly taken as verifications o f the "rigid-pore" concept, are now interpret­ ed in terms o f four factors responsible for sizediscrimination: collision frequency, duration o f sin­ gle contacts, size-dependent frictional force, and the extent o f cooperation am ong fibres, due to crosslinking and to simultanous contacts o f several fibres to a single macro-ion. Som e functions rel­ evant for problem s o f molecular weight determ ination by gel electrophoresis are discussed in rela­ tion to the suggested model. 
  Reference    Z. Naturforsch. 34c, 512—528 (1979); received March 22 1979 
  Published    1979 
  Keywords    Polyacrylamide, M olecular Sieve, Electrophoresis, Viscosity, Frictional Coefficient, Partition C oefficient 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0512.pdf 
 Identifier    ZNC-1979-34c-0512 
 Volume    34