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'Electron Microscopy' in keywords Facet   section ZfN Section C:Volume 034  [X]
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1979 (2)
1Author    W. Schröder, H. Stieve, I. Classen-LinkeRequires cookie*
 Title    Accessibility of Extracellular Space in the Rhabdome of the Living Isolated Retina of the Crayfish  
 Abstract    Diffusion of the tracer enzyme horseradish peroxidase (HRP) into the extracellular spaces of living isolated crayfish retinas was monitored by light-and electronmicroscopic identification of the reaction product obtained after immobilisation of the enzyme by fixation. During the incuba­ tion in enzyme containing saline (up to 3 hours) the extracellularly recorded electroretinogram (ERG) stayed normal and showed no substantial change. The reaction product of the enzyme HRP was found only extracellularly in the space between the microvilli in the rhabdome. The results indicate an exchange of substances via extracellular pathways between the extra­ cellular side of the photosensory membrane and the bathing fluid in the living isolated crayfish retina. 
  Reference    Z. Naturforsch. 34c, 136 (1979); received November 7 1978 
  Published    1979 
  Keywords    Light Microscopy, Electron Microscopy, Horseradish Peroxidase Tracer Labelling, Electroretinogram, Retina, Extracellular Compartments 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0136.pdf 
 Identifier    ZNC-1979-34c-0136 
 Volume    34 
2Author    OndinaD. Colman, JuanC. StockertRequires cookie*
 Title    Electron Microscopy of Synaptonemal Complexes in Semithin Sections  
 Abstract    The technique of osmium tetroxide fixation followed by a treatment with p-phenylenediamine was applied to mouse testes in order to analyze pachytene nuclei. Observation of Epon semithin sections (0.25 — 0.5 um) in conventional elec­ tron microscopy revealed the presence of positively stained synaptonemal complexes surrounded by unstained chromatin. The present results show that by using this method a suitable contrast and resolution can be achieved for semithin sections, which facilitates the topographical study of syn­ aptonemal complexes. 
  Reference    Z. Naturforsch. 34c, 299 (1979); received December 11 1978 
  Published    1979 
  Keywords    Synaptonemal Complex, Osmium/p-Phenylenediamine, Mouse Spermatocytes, Semithin Sections, Electron Microscopy 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0299_n.pdf 
 Identifier    ZNC-1979-34c-0299_n 
 Volume    34