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1	Author	Z. Naturforsch	Requires cookie*
	Title	Isolierung und Morphologische Charakterisierung des Kartoffelblattrollvirus (PLRV)
	Abstract	Isolation and M orphological Characterization o f the Potato Leafroll Virus (PLRV) J. B lessing Virologische Abteilung des Instituts für Hygiene und M ikrobiologie der U niversität des Saarlandes. H om burg/Saar, und S. S a rk a r In spite of its very low concentration, the potato leafroll virus (PLRV) can be enriched from leaf-homogenates of the infected host plant Physalis floridana by a series of separation steps and purified by ultracentrifugation through layers of cesium chloride of defined density. U nder the present experimental conditions the virus accumulates as an invisible band in a region where the density of CsCl is 1.39 ± 0.01 g/cm 3. In negatively stained preparations, the virions show a diam eter of 23 nm and some morphological criteria indicate an icosahedral symmetry.
	Reference	Z. Naturforsch. 36c, 884—887 (1981); received May 23 1979/May 18 1981
	Published	1981
	Keywords	Potato Virus, Icosahedron, Electron Microscopy
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	TEI-XML for	default:Reihe_C/36/ZNC-1981-36c-0884.pdf
	Identifier	ZNC-1981-36c-0884
	Volume	36

2	Author	Srabani Banerjee, S. N. Chatterjee	Requires cookie*
	Title	Effect of Sodium Cholate on the Phase Transition Temperature of Dipalmitoyl Phosphatidylcholine
	Abstract	On treatm ent with sodium cholate, the phase transition tem perature (Tc) of m ultilam ellar liposomes derived from dipalm itoyl phosphatidylcholine (DPPC) progressively decreased with increasing cholate: lipid-P m olar ratio. A molar ratio of 4.5 caused a one degree depression in Tc. The unilamellar cholate vesicles o f DPPC exhibited the same Tc as that o f the m ultilam ellar ones. The formation of unilam ellar vesicles from the m ultilam ellar ones by cholate treatm ent was in vestigated by electron microscopy.
	Reference	Z. Naturforsch. 38c, 302—306 (1983); received Septem ber 20/N ovem ber 15 1982
	Published	1983
	Keywords	Liposomes, DPPC, Sodium Cholate, Phase Transition, Electron Microscopy
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	TEI-XML for	default:Reihe_C/38/ZNC-1983-38c-0302.pdf
	Identifier	ZNC-1983-38c-0302
	Volume	38

3	Author	EgbertJ. Boekema, Günter Schmidt, Peter Gräber, JanA. Berden	Requires cookie*
	Title	Structure of the ATP-Synthase from Chloroplasts and Mitochondria Studied by Electron Microscopy
	Abstract	The structure of the ATP-synthase, F 0 F,, from spinach chloroplasts and beef heart mitochon-dria has been investigated by electron microscopy with negatively stained specimens. The deter-gent-solubilized ATP-synthase forms string-like structures in which the F 0 parts are aggregated. In most cases, the F, parts are arranged at alternating sides along the string. The F 0 part has an approximate cylindrical shape with heights of 8.3 and 8.9 nm and diameters of 6.2 and 6.4 nm for the chloroplast and mitochondrial enzyme, respectively. The F, parts are disk-like structures with a diameter of about 11.5 nm and a height of about 8.5 nm. The F, parts are attached to the strings, composed of F n parts, in most cases, with their smallest dimension parallel to the strings. The stalk connecting F 0 and F, has a length of 3.7 nm and 4.3 nm and a diameter of 2.7 nm and 4.3 nm for the chloroplast and mitochondrial enzyme, respectively.
	Reference	Z. Naturforsch. 43c, 219—225 (1988); received December 1 1987
	Published	1988
	Keywords	ATP-Synthase, Enzyme Structure, Electron Microscopy
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	TEI-XML for	default:Reihe_C/43/ZNC-1988-43c-0219.pdf
	Identifier	ZNC-1988-43c-0219
	Volume	43

4	Author	Ulrich Schraermeyer, Hennig Stieve, Michael Rack	Requires cookie*
	Title	Cytochemical Localization of Guanylate and Adenylate Cyclase in Photoreceptor Cells of the Fly
	Abstract	In photoreceptor cells o f invertebrates light triggers an enzyme cascade in which the phos-phoinositide pathway is crucially involved. Likewise, there is growing evidence of an impor tant role of cyclic nucleotides, too. To localize these enzymes able to catalyze the formation of cGM P and cAMP, the spatial distribution of guanylate cyclase (EC 4.6.1.2) and adenylate cyclase (EC 4.6.1.1) was determined in photoreceptor cells of the fly. In photoreceptor cells of the blowfly (Calliphora erythrocephala), the electron dense reaction product o f guanylate cyclase was found within the phototransducing region, the rhabdomeral microvilli and in the mitochondria. Staining was also observed throughout the cytoplasm of the microvilli. With the same cytochemical method, reaction product for adenylate cyclase was found on the tips of the photosensory membrane, and not in the cytoplasm o f the rhabdomeral microvilli. The results presented here further argue for an important role of one or possibly two cyclic nucleotides in the photoreceptor cells, and possibly in the process o f phototransduction of in vertebrates.
	Reference	Z. Naturforsch. 50c, 695—6 (1995); received May 24/July 10 1995
	Published	1995
	Keywords	Electron Microscopy, Guanylate Cyclase, Adenylate Cyclase, Photoreceptor, Fly
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	TEI-XML for	default:Reihe_C/50/ZNC-1995-50c-0695.pdf
	Identifier	ZNC-1995-50c-0695
	Volume	50

5	Author	L. Raeymaekers	Requires cookie*
	Title	The Sarcoplasmic Reticulum of Smooth M uscle Fibers
	Abstract	The ability of the sarcoplasm ic (endoplasmic) reticulum (SR, ER) of smooth muscle cells to accumulate Ca was dem onstrated by measuring the uptake of 45Ca in fibers which were chemically skinned with saponin, and by electron cytochemistry of the accum ulated Ca. The Ca uptake was dependent on ATP and it was stim ulated by oxalate, as it is the case in SR of striated muscle. Electron microscopy of the skinned smooth muscle preparations revealed the presence o f calcium oxalate deposits in the reticulum . The SR vesicles were isolated from several sm ooth muscles. The purification was carried out by taking advantage o f the density increase o f the SR vesicles after loading with calcium in the presence of oxalate. Among the muscles investigated the smooth muscle of the pig stomach was found to be the most suitable and it was selected for further biochem ical and m orphological characterization of the SR vesicles. These vesicles, which contain calcium oxalate crystals, were able to accumulate an additional am ount of Ca. The Ca uptake was supported by several energy yielding substrates. T h eir order o f potency was ATP > dATP ~ U TP > ITP > G TP ~ CTP. The rate of Ca uptake was two orders o f m agnitude slower than that in SR of skeletal muscle. The measurement of the level o f phosphorylated Ca transport interm ediate showed that this difference is due to smaller num ber of calcium transport sites per vesicle. The distribution o f intram em brane particles in freeze-fractured specimens is in agreem ent with this conclusion.
	Reference	Z. Naturforsch. 37c, 481—488 (1982); received January 25 1982
	Published	1982
	Keywords	Smooth Muscle, Microsomes, Sarcoplasmic Reticulum, Calcium Metabolism, Electron Microscopy
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	TEI-XML for	default:Reihe_C/37/ZNC-1982-37c-0481.pdf
	Identifier	ZNC-1982-37c-0481
	Volume	37

6	Author	DrBernhard Wolf	Requires cookie*
	Title	Elektronen-Energie-Verlust-Spektroskopische Untersuchungen an Luftfiltern zur Bewertung der Luftqualität Electron-Energy-Loss-Spectroscopical Investigations on Air Filters for the Analysis of the Air Quality
	Abstract	In trace analysis it is more and more attempted to replace wet-chemical detection procedures by methods which allow a quantitative analysis without any material disintegration, but on exploiting the characteristic physical properties of the components searched for. Based on a procedure for physical and biological valuation of the air quality by means of X-ray microanalysis (WDX/EDX), a procedure which was previously developed by our group, we deepened our investigations by the help of electron-energy-loss-spectroscopy (EELS). The combination of that procedure with EELS proved to be very advantageous as it revealed a high sensitivity as well as a high energy resolution. The main advantages are to be found in the simple arrangement of the sample detector and in the fact, not only being able to examine deposits microscopically, but also to analyze them chemically without disintegrating the material. Thus loss of material and denaturation are largely excluded.
	Reference	Z. Naturforsch. 43c, 155—161 (1988); received November 4 1987
	Published	1988
	Keywords	Air Analysis, Filter Detection, Electron Microscopy, Electron-Energy-Loss-Spectroscopy
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	TEI-XML for	default:Reihe_C/43/ZNC-1988-43c-0155.pdf
	Identifier	ZNC-1988-43c-0155
	Volume	43

7	Author	Ulrich Schraermeyer	Requires cookie*
	Title	Evidence for a Pathway of Distal Screening Pigment Granules across the Basement Membrane of the Crayfish Photoreceptor
	Abstract	The distal pigment cells o f Orconectes limosus contain two layers o f large electron lucent vacuoles that are separated by layers o f small right-angled platelets adjacent to the crystalline cones. The crystalline cones o f the dioptric apparatus o f this species have evaginations into the distal pigment cell cytoplasm. In photoreceptors o f Orconectes limosus and Procambarus clar-kii a dark pigment accumulation site was detected just distal to the basement membrane at the edges o f each retina. These pigment accumulations occurred independent o f the state o f light adaptation. Ultrastructurally the pigment granules at this accumulation site resemble distal screening pigment granules according to their size (up to 1.2 jim in diameter) and fibrous structure. Distal screening pigment granules were also found in tube-like cell processes or ex-tracellularly within and proximal to the retinal basement membrane, indicating pigment trans port to and across the basement membrane. Proximal to the basement membrane screening pigment granules were also observed disintegrated to a gravel-like electron dense material in widely branched cells. Evidence was found that an electron dense material, probably resulting from disintegrating screening pigment granules, was incorporated in the integument o f the eyestalk. Four hours after injection o f gold particles into the eye stalk distal to the retina they were detected inside and proximal to the retinal basement membrane.
	Reference	Z. Naturforsch. 47c, 453 (1992); received June 13 1991/February 11 1992
	Published	1992
	Keywords	Electron Microscopy, Crayfish, Distal Screening Pigment Granules, Basement Membrane, Crystalline Cone
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	TEI-XML for	default:Reihe_C/47/ZNC-1992-47c-0453.pdf
	Identifier	ZNC-1992-47c-0453
	Volume	47

8	Author	Riidiger Riehl	Requires cookie*
	Title	Eine A Special Attaching-Mechanism between the Cortex radiatus externus and the Follicle Epithelium of the Oocytes of Gobio gobio
	Abstract	besondere Haftvorrichtung zwischen Cortex radiatus externus und Follikel epithel der Oocyten von G obio g o b io (L). The cortex radiatus externus and the follicle epithelium of the freshwater teleost Gobio gobio are firmly attached together by a special attaching-mechanism. The apical surface of the plugs of the cortex radiatus externus has recesses and excavations which fit in the short projections of the follicle cells and prevent moving of these cells on the egg mem branes. This attaching-mechanism also assists in fastening the oocytes (eggs) on the substrate.
	Reference	(Z. Naturforsch. 31c, 628 [1976]; eingegangen am 23. Juni 1976)
	Published	1976
	Keywords	Electron Microscopy, Gobio gobio, Oocytes, Cortex radiatus externus, Attaching-Mechanism
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	TEI-XML for	default:Reihe_C/31/ZNC-1976-31c-0628_n.pdf
	Identifier	ZNC-1976-31c-0628_n
	Volume	31

9	Author	Marvin Stromer, Wilhelm Hasselbadh	Requires cookie*
	Title	Fusion of Isolated Sarcoplasmic Reticulum Membranes
	Abstract	Fragmented sarcoplasmic reticulum (FSR) vesicles from rabbit muscle were suspended in 1.5 — 5% glycerol solutions and were pelleted onto aluminum foil disks in a modified centrifuge tube. Examination of these pellets in the electron microscope after drying for 2 — 2.5, 4 — 5.5, and 21 hours revealed a progression of changes. First, distances between individual, round vesicles decreases. Next, somewhat flattened vesicles establish limited areas of contact with adjacent vesicles. Finally, vesicle fusion occurs and extended areas of double bilayers are formed. A water loss-time interaction appears to be needed for the fusion process. A Hg-phenyl azoferritin com pound was used as a marker to identify intra-and extra-vesicular space in the fused samples. Quantitative measurements of birefringence during imbibition of pellet slices in a graded series of glycerol solutions indicates a steadily increasing amount of birefringence until 60 — 80% glycerol (»/ = 1.41 —1.43) is readied. The plateau seen in this part of the curve is again followed by steadily increasing birefringence at higher glycerol concentrations. This interruption in the birefringence curve is presumably due to a matching of the refractive indices of the glycerol solution and a lipid component in the membranes.
	Reference	(Z. Naturforsch. 31c, 703 [1976]; received August 23 1976)
	Published	1976
	Keywords	Membrane Fusion, Sarcoplasmic Reticulum, Glycerol, Electron Microscopy, Polarizing Microscopy
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	TEI-XML for	default:Reihe_C/31/ZNC-1976-31c-0703.pdf
	Identifier	ZNC-1976-31c-0703
	Volume	31

10	Author	U. P. Fringeli	Requires cookie*
	Title
	Reference	(Z. Naturforsch. 31c, 746 [1976]; received August 17 1976)
	Published	1976
	Keywords	Electron Microscopy, Radiation Damage, Polypeptide Monolayers, Infrared Dichroism, Conformational Changes
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	TEI-XML for	default:Reihe_C/31/ZNC-1976-31c-0746_n.pdf
	Identifier	ZNC-1976-31c-0746_n
	Volume	31

11	Author	Riidiger Riehl	Requires cookie*
	Title	Parakristalline Körper in jungen Oocyten der Schmerle
	Abstract	N o em a ch eilu s barbatulus (L.) (Teleostei, Cobitidae) Paracristalline Bodies in Young Oocytes of the Loach, Noemacheilus barbatulus (L.) (Teleostei, Cobitidae) Young oocytes of the loach, Noemacheilus barbatulus (L.), were studied by electron microscopy. Fistular paracristalline bodies are found in the oocytes during state I. They consist of many osmiophilic particles with a diameter of 150 — 300 A. Ultrahistochemical experiments have demonstrated that the paracristalline bodies are mainly composed of pro teins. The paracristalline bodies are yolk precursors.
	Reference	(Z. Naturforsch. 32c, 305 [1977]; eingegangen am 26. Januar 1977)
	Published	1977
	Keywords	Paracristalline Bodies, Oocytes, Yolk Precursors, Electron Microscopy, Noemacheilus barbatulus
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	TEI-XML for	default:Reihe_C/32/ZNC-1977-32c-0305_n.pdf
	Identifier	ZNC-1977-32c-0305_n
	Volume	32

12	Author	Erhard Schulte, Rüdiger Riehl	Requires cookie*
	Title	Elektronenmikroskopische Untersuchungen am Geruchsorgan von Ophicephalus
	Abstract	The regio olfactoria of Ophicephalus was studied by transmission electron microscopy. Two types of receptor cells were found: ciliary receptor cells and microvillous re ceptor cells. Both of these types represent primary sensory cells. So one can regard the light microscopical findings of Kapoor and Ojha as disproved.
	Reference	(Z. Naturforsch. 32c, 307—308 [1977]; received January 20 1977)
	Published	1977
	Keywords	Sensory Cells, Olfactory Organ, Electron Microscopy, Ophicephalus obscurus, Teleostei
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	TEI-XML for	default:Reihe_C/32/ZNC-1977-32c-0307_n.pdf
	Identifier	ZNC-1977-32c-0307_n
	Volume	32

13	Author	Jürgen Kreft	Requires cookie*
	Title	Reovirus-Specific Messenger Ribonucleoprotein Particles from Heia Cells
	Abstract	When reovirus-infected Heia cells are incubated at 43 °C virus-specific messenger RNA is released from the polysomes. It accumulates free in the cytoplasm as messenger ribonucleopro tein particles (mRNPs). These particles have a sedimentation rate of about 50 S and a buoyant density in CsCl of 1.42 g/cm 3. Reovirus mRNPs contain, besides all three size classes o f reovirus messenger RNA, the same spectrum of proteins found in the polysomal mRNPs from uninfected cells, plus two additional proteins with molecular masses of 70000 d and 110000 d, respectively. Electron microscopic examination of the reovirus structures with a total mean length of 0.5 nm.
	Reference	Z. Naturforsch. 35c, 1046—1051 (1980); received May 14/August 13 1980
	Published	1980
	Keywords	Heia Cells, Reovirus, Messenger Ribonucleoprotein Particles, mRNP-Proteins, Electron Microscopy
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	TEI-XML for	default:Reihe_C/35/ZNC-1980-35c-1046.pdf
	Identifier	ZNC-1980-35c-1046
	Volume	35

14	Author	P. V. Monje3, E. J. Baranb	Requires cookie*
	Title	On the Formation of Whewellite in the Cactaceae Species Opuntia microdasys
	Reference	Z. Naturforsch. 52c, 267—269 (1997); received January 20 1997
	Published	1997
	Keywords	Cactaceae, Opuntia microdasys, Whewellite, Isolation, Electron Microscopy, Infrared Spectrum
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	TEI-XML for	default:Reihe_C/52/ZNC-1997-52c-0267_n.pdf
	Identifier	ZNC-1997-52c-0267_n
	Volume	52

15	Author	H. Wesch, R. Jonak, H. Nemetschek-Gansler, H. Riedl, Th Nemetschek	Requires cookie*
	Title	Der Einfluß von D-Penicillamin auf den Gehalt einiger Organe an Spurenelementen sowie auf die mechanischen Eigenschaften von Kollagen Trace Elements in Several Organs and Mechanical Properties of Collagen under the Influence of D-Penicillamin
	Abstract	The content of trace elements in several organs of rats under the influence of D-penicillamine (D-PA) was investigated by the neutronactivation-analysis. It could be shown an diminution of Cu, and Co under D-PA-treatment, the content of Fe, Mn, Rb and Zn was not influenced. The investigat ed organs didn't show any submicroscopic alterations under D-PA. On isolated collagen fibrils of tail tendon was seen a significantly diminuition of E-moduls. In accordance with Siegel the principal effect of D-PA is thought to block the synthesis of functional groups from Schiff-base crosslink precursors but not to inhibit lysyloxidase by loss of Cu-ions of connective tissue. The thermostability of D-PA influenced fibrils is changed in stretched state only and will be due to the lack of crosslink Schiff-bases; where as the shrinking point of not stretched fibrils shows only aging dependent changes.
	Reference	Z. Naturforsch. 33c, 346 (1978); eingegangen am 17. März 1978
	Published	1978
	Keywords	D-Penicillamin, Trace Elements, Electron Microscopy, Biosynthesis, Cross-Links, Mechanical Properties of Collagen
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	TEI-XML for	default:Reihe_C/33/ZNC-1978-33c-0346.pdf
	Identifier	ZNC-1978-33c-0346
	Volume	33

16	Author	W. Schröder, H. Stieve, I. Classen-Linke	Requires cookie*
	Title	Accessibility of Extracellular Space in the Rhabdome of the Living Isolated Retina of the Crayfish
	Abstract	Diffusion of the tracer enzyme horseradish peroxidase (HRP) into the extracellular spaces of living isolated crayfish retinas was monitored by light-and electronmicroscopic identification of the reaction product obtained after immobilisation of the enzyme by fixation. During the incuba tion in enzyme containing saline (up to 3 hours) the extracellularly recorded electroretinogram (ERG) stayed normal and showed no substantial change. The reaction product of the enzyme HRP was found only extracellularly in the space between the microvilli in the rhabdome. The results indicate an exchange of substances via extracellular pathways between the extra cellular side of the photosensory membrane and the bathing fluid in the living isolated crayfish retina.
	Reference	Z. Naturforsch. 34c, 136 (1979); received November 7 1978
	Published	1979
	Keywords	Light Microscopy, Electron Microscopy, Horseradish Peroxidase Tracer Labelling, Electroretinogram, Retina, Extracellular Compartments
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	TEI-XML for	default:Reihe_C/34/ZNC-1979-34c-0136.pdf
	Identifier	ZNC-1979-34c-0136
	Volume	34

17	Author	OndinaD. Colman, JuanC. Stockert	Requires cookie*
	Title	Electron Microscopy of Synaptonemal Complexes in Semithin Sections
	Abstract	The technique of osmium tetroxide fixation followed by a treatment with p-phenylenediamine was applied to mouse testes in order to analyze pachytene nuclei. Observation of Epon semithin sections (0.25 — 0.5 um) in conventional elec tron microscopy revealed the presence of positively stained synaptonemal complexes surrounded by unstained chromatin. The present results show that by using this method a suitable contrast and resolution can be achieved for semithin sections, which facilitates the topographical study of syn aptonemal complexes.
	Reference	Z. Naturforsch. 34c, 299 (1979); received December 11 1978
	Published	1979
	Keywords	Synaptonemal Complex, Osmium/p-Phenylenediamine, Mouse Spermatocytes, Semithin Sections, Electron Microscopy
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	TEI-XML for	default:Reihe_C/34/ZNC-1979-34c-0299_n.pdf
	Identifier	ZNC-1979-34c-0299_n
	Volume	34