| | 1 | Author
| W. Nikolaus Kiihn-Velten | Requires cookie* | | | Title
| Effects of Compatible Solutes on Mammalian Cytochrome P450 Stability  | | | | Abstract
| The sensitivity of pig cytochrome P450cl7 (CYP17), an endoplasmic reticulum membrane-bound enzyme, towards heat denaturation (48 °C) was measured by the P450-to-P420 spectral transition indicating conforma tional labilization of the protein. Both sucrose and glu cose have comparable and increasingly protective effects at concentrations ranging from 100 to 800 mM, while ec toine, a novel zwitterionic compatible solute which regu lates bacterial osmoadaptation and stabilizes cyto plasmic enzymes, has a strong labilizing effect on CYP17 and favours its proteolytic inactivation possibly by elec trostatic derangements. Sucrose or glucose, but not ec toine, can therefore eventually be proposed as compati ble stabilizers of cytochrome P450 structures. | | | |
Reference
| Z. Naturforsch. 52c, 132—135 (1997); received September 18/November 18 1996 | | | |
Published
| 1997 | | | |
Keywords
| Cytochrome P450 Protein Stability, Osmolytes, Ectoine, Sucrose | | | |
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| default:Reihe_C/52/ZNC-1997-52c-0132_n.pdf | | | | Identifier
| ZNC-1997-52c-0132_n | | | | Volume
| 52 | |
| 2 | Author
| Willi Schuh, Heinrich Puff, ErwinA. Galinski, HansG. Trüper | Requires cookie* | | | Title
| Die Kristallstruktur des Ectoin, einer neuen osmoregulatorisch wirksamen Aminosäure The Crystal Structure of Ectoine, a Novel Amino Acid of Potential Osmoregulatory Function | | | | Abstract
| The crystal structure of ectoine (l,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid) iso lated from the extremely halophilic phototrophic bacterium Ectothiorhodospira halochloris was determined by X-ray analysis. This novel cyclic amino acid forms zwitterionic molecules with delocalized ^-bonding in the N-C-N group of the hydrated pyrimidine ring. The molecules are connected by N -H ...O hydrogen bonds. The excellent solubility of ectoine in water can be explained by its structural features which also account for its function as a compatible solute in halophilic bacteria. | | | |
Reference
| Z. Naturforsch. 40c, 780—784 (1985); received July 8/August 9 1985 | | | |
Published
| 1985 | | | |
Keywords
| gewidmet X-Ray Structure Analysis, Ectoine, Amino Acid, Compatible Solute, Osmoregulation | | | |
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| default:Reihe_C/40/ZNC-1985-40c-0780.pdf | | | | Identifier
| ZNC-1985-40c-0780 | | | | Volume
| 40 | |
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