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'Ectoine' in keywords
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1997 (1)
1985 (1)
1Author    W. Nikolaus Kiihn-VeltenRequires cookie*
 Title    Effects of Compatible Solutes on Mammalian Cytochrome P450 Stability  
 Abstract    The sensitivity of pig cytochrome P450cl7 (CYP17), an endoplasmic reticulum membrane-bound enzyme, towards heat denaturation (48 °C) was measured by the P450-to-P420 spectral transition indicating conforma­ tional labilization of the protein. Both sucrose and glu­ cose have comparable and increasingly protective effects at concentrations ranging from 100 to 800 mM, while ec­ toine, a novel zwitterionic compatible solute which regu­ lates bacterial osmoadaptation and stabilizes cyto­ plasmic enzymes, has a strong labilizing effect on CYP17 and favours its proteolytic inactivation possibly by elec­ trostatic derangements. Sucrose or glucose, but not ec­ toine, can therefore eventually be proposed as compati­ ble stabilizers of cytochrome P450 structures. 
  Reference    Z. Naturforsch. 52c, 132—135 (1997); received September 18/November 18 1996 
  Published    1997 
  Keywords    Cytochrome P450 Protein Stability, Osmolytes, Ectoine, Sucrose 
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 TEI-XML for    default:Reihe_C/52/ZNC-1997-52c-0132_n.pdf 
 Identifier    ZNC-1997-52c-0132_n 
 Volume    52 
2Author    Willi Schuh, Heinrich Puff, ErwinA. Galinski, HansG. TrüperRequires cookie*
 Title    Die Kristallstruktur des Ectoin, einer neuen osmoregulatorisch wirksamen Aminosäure The Crystal Structure of Ectoine, a Novel Amino Acid of Potential Osmoregulatory Function  
 Abstract    The crystal structure of ectoine (l,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid) iso­ lated from the extremely halophilic phototrophic bacterium Ectothiorhodospira halochloris was determined by X-ray analysis. This novel cyclic amino acid forms zwitterionic molecules with delocalized ^-bonding in the N-C-N group of the hydrated pyrimidine ring. The molecules are connected by N -H ...O hydrogen bonds. The excellent solubility of ectoine in water can be explained by its structural features which also account for its function as a compatible solute in halophilic bacteria. 
  Reference    Z. Naturforsch. 40c, 780—784 (1985); received July 8/August 9 1985 
  Published    1985 
  Keywords    gewidmet X-Ray Structure Analysis, Ectoine, Amino Acid, Compatible Solute, Osmoregulation 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0780.pdf 
 Identifier    ZNC-1985-40c-0780 
 Volume    40