| | 1 | Author
| Peter Mascheck, Klaus Scheller, Peter Karlson | Requires cookie* | | | Title
| Changes in Gene Expression during Larval Development of Calliphora vicina Induced by Ecdysterone  | | | | Abstract
| The influence of the insect hormone ecdysterone on the composition of the RNA from the fat bodies of C alliph ora larvae was investigated by hybridization experiments. DNA was iodinated in vitro with 125J. In saturation experiments, total RNA was hybridized to total DNA; under similar conditions, poly (A) RNA was hybridized to unique DNA sequences. RNA isolated from animals treated with ecdysterone exhibits a higher percentage of hybridization than a corresponding control RNA from the same developmental stage, a result even more prominent when poly (A) RNA was hybridized with unique DNA. It is concluded that ecdysterone induces the transcription of new unique DNA sequences. | | | |
Reference
| (Z. Naturforsch. 32c, 434 [1977]; received December 27 1976) | | | |
Published
| 1977 | | | |
Keywords
| Gene Expression, Insect Hormones, Ecdysterone, Larval Development, DNA-RNA Hybridization | | | |
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| default:Reihe_C/32/ZNC-1977-32c-0434.pdf | | | | Identifier
| ZNC-1977-32c-0434 | | | | Volume
| 32 | |
| 2 | Author
| Klaus Scheller, Peter Karlson, Dietrich Bodenstein | Requires cookie* | | | Title
| Effects of Ecdysterone and the Juvenile Hormone Analogue Methoprene on Protein, R N A and D N A Synthesis in Wing Discs of Calliphora vicina | | | | Abstract
| Adolf Butenandt zum 75. G ebu rtstag gew idm et The protein, RNA and DNA content of wing discs increase exponentially during the last larval instar. Biosynthesis of protein, RNA and DNA was studied by injecting labelled precursors into larvae or white prepupae and measuring the incorporation in the wing discs dissected out after an appropriate time. Protein synthesis is stimulated by ecdysterone, methoprene has no effect. Bio synthesis of rRNA is increased in wing discs of white prepupae after ecdysterone or methoprene injection. Methoprene inhibits the synthesis of mRNA, while ecdysterone has no clear-cut effect within the limits of our method (gel electrophoretic analysis). Ecdysterone and methoprene have no detectable influence on incorporation of thymidine into DNA, but the incorporation label from uridine into DNA is diminished; this effect may be due to changes in the precursor pool. | | | |
Reference
| Z. Naturforsch. 33c, 253 (1978); received March 9 1978 | | | |
Published
| 1978 | | | |
Keywords
| Ecdysterone, Juvenile Hormone, Wing Discs, Protein Synthesis, RNA Synthesis, DNA Synthesis, C alliph ora vicina | | | |
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| default:Reihe_C/33/ZNC-1978-33c-0253.pdf | | | | Identifier
| ZNC-1978-33c-0253 | | | | Volume
| 33 | |
| 3 | Author
| B. M. Aršálek, M. Šimek, R. J. Smith | Requires cookie* | | | Title
| The Effect of Ecdysterone on the Cyanobacterium Nostoc 6720 | | | | Abstract
| The insect hormone ecdysterone stimulated dinitrogen fixation, heterocyst formation, cell size and protein synthesis in suspension cultures of the cyanobacterium Nostoc 6720 within 48 h when added to the culture at concentrations between 10-9 and 10-7 M . In the presence of the hormone the volume of vegetative cells increased by 6-fold and that of heterocysts by near ly twice as compared to untreated controls. All the observed effects were inhibited by the cal-cium-calmodulin inhibitor, trifluoperazine. Cholesterol used as a null control had no signifi cant effect. | | | |
Reference
| Z. Naturforsch. 47c, 726—730 (1992); received May 26/July 27 1992 | | | |
Published
| 1992 | | | |
Keywords
| Cyanobacterium, Nostoc PCC 6720, Ecdysterone, Enhanced Nitrogen Fixation, Cell Size, Protein Synthesis | | | |
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| default:Reihe_C/47/ZNC-1992-47c-0726.pdf | | | | Identifier
| ZNC-1992-47c-0726 | | | | Volume
| 47 | |
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