1 | Author | M. Valentin, W. E. Bollenbacher, L. I. Gilbert, H. Kroeger | Requires cookie* |
Title | Alterations in Ecdysone Content during the Post-Embryonic Development of Chironomus thummi: Correlations with Chromosomal Puffing ![]() | ||
Abstract | The ecdysone titer o f larvae and pupae o f Chironomus thum m i was determined by radioimmune assay (R IA) and revealed a concentration of about 150 ng/g fresh weigth in late third instar larvae and a peak of more than 450 ng/g fresh weight just preceding pupation. The ecdysone titer curve shows a high degree o f correlation with previously observed puffing activity at the ecdysone sensitive chromosomal site I l l d l . Further, //-ecdysone is the only endogenous ecdysone detected by high pressure liquid chromatography during larval-pupal development. It was also observed that developmentally arrested Chironomus contain less ecdysone than " normal" larvae. | ||
Reference | Z. Naturforsch. 33c, 557 (1978); received June 5 1978 | ||
Published | 1978 | ||
Keywords | Insect Hormones, Ecdysteroids, Chromosomal Puffing, Chironomus thum mi | ||
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