| | 1 | Author
| Lutz Reum, G. Erd Käuser, U. Lrike Enderle, Jan Koolman | Requires cookie* | | | Title
| A Steroid-Binding Protein from Insect Haemolymph Isolated by PhotoafTinity Labelling and Immunoadsorption  | | | | Abstract
| Endogenous ecdysteroids as well as radiolabelled exogenous ecdysone were crosslinked to a protein when haemolymph from blowfly larvae was irradiated with UV-light under optimized conditions. This indicates that larval haemolymph of blowflies contains an ecdysteroid-binding protein. The crosslinked ecdysteroid-protein complex was shown to form a ternary complex when mixed with ecdysteroid-specific antibodies. Formation of the ternary complex was due to a specific interaction of the binding sites of the antibodies with the steroid. This specific interaction was used to isolate the ecdysteroid-protein complex from irradiated crude larval haemolymph by immunoadsorption. The method described here represents a rapid approach for the isolation o f steroid-binding proteins by a combination o f photoaffinity labelling and immunoadsorption. | | | |
Reference
| Z. Naturforsch. 37c, 967—9 (1982); received March 11/June 8 1982 | | | |
Published
| 1982 | | | |
Keywords
| Immunoadsorption, Ecdysteroids, Calliphorin, Steroid-Binding Protein, Photoaffinity-Labelling | | | |
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| default:Reihe_C/37/ZNC-1982-37c-0967.pdf | | | | Identifier
| ZNC-1982-37c-0967 | | | | Volume
| 37 | |
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