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1989 (1)
1982 (2)
1Author    E. I. Kukko, J. K. HeinonenRequires cookie*
 Title    Effect o f P en icillins on the Level o f Inorganic Pyrophosphatase in Escherichia coli K 12  
 Abstract    The relative rate o f DNA synthesis increases over control level and the synthesis o f inorganic pyrophospha­ tase is stimulated when cells o f E. coli are cultured in the presence of penicillins which inhibit cell division and cause filamentation o f the cells. In contrast to that in temperature sensitive cell division mutants filamentation at 42 °C does not change the D N A /cell mass ratio and the synthesis of inorganic pyrophosphatase is not stimulated. This suggests that the change in the synthesis of the enzyme is not correlated with the filamentation but is a consequence of the supernormal D N A /cell mass ratio that appears in the presence of penicillins. 542 
  Reference    Z. Naturforsch. 37c, 542 (1982); received December 28 1981 
  Published    1982 
  Keywords    Penicillin, Inorganic Pyrophosphatase, E coli 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0542_n.pdf 
 Identifier    ZNC-1982-37c-0542_n 
 Volume    37 
2Author    K. Grossmann, H. Bisswanger, H. U. SeitzRequires cookie*
 Title    Differential Fluorescence and Kinetic Studies on the Template-Binding of RNA Polymerase from Parsley and Escherichia coli  
 Abstract    A fluorescence spectroscopy m ethod is described for studying association o f RNA polymerase with DNA templates. Using double beam differential fluorescence at excitation and emission wavelengths of 285 and 335 nm, respectively, the new technique discriminates non-specific decrease of fluorescence intensity by addition o f D N A from quenching of polymerase fluorescence by protein-nucleic acid interactions. C om paring the results with studies of UM P incorporation into RNA, the Äg-values o f tem plate-binding were in good agreem ent with the values for RNA synthesis, pointing to specific interaction o f polym erase and the D NA tem plate measured by the fluorescence method. W hile E. coli enzyme showed higher affinity for tem plates such as heat-denatured poly [d(A-T)] and poly [d(G -C)] parsley RNA polym erase I accepted such templates with the same affinity as salmon sperm DNA. It is obvious that divalent cations are not necessary for the interaction of both enzymes with single-stranded DNA -tem plates. UM P incorporation studies suggest that transcription is a cooperative process. 
  Reference    Z. Naturforsch. 37c, 81—86 (1982); received O ctober 261981 
  Published    1982 
  Keywords    RNA Polymerase, Template-Binding, D ifferential Fluorescence, Parsley, E coli 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0081.pdf 
 Identifier    ZNC-1982-37c-0081 
 Volume    37 
3Author    D. D. Enklau, R. Stahl, W. KöhnleinRequires cookie*
 Title    Wirkung von Neocarzinostatin auf E. co/i-Mutanten mit defekter DNA-Reparatur The Influence of Neocarzinostatin on the Colony Forming Ability of E. coli Mutants Deficient in DNA R epair  
 Abstract    The colony forming ability of E. coli m utants defective in D N A repair was com pared to that of the parent strain AB 1157 after neocarzinostatin treatm ent. A recA and a recB m utant were most sensitive. The suppression of the recB m utation in the recBC sbcBC m utant, which is as sensitive as the parent strain, indicates that recB is not the primary pathw ay by which lesions after NCS treatm ent are repaired. The survival curve of the recBC recF sbcBC m utant, corresponding to that of the recF m utant, further supports this interpretation. The relative resistance of the recBC recF sbcBC mutant suggests that NCS lesions are not only repaired by the recF and recB pathway. An alternative pathway could be the SOS induction, as a lexA m utant also is sensitive to NCS. The sensitivity of the uvrA and polA xthA m utants, however is explained by the involvement of the uvrA and polA gen products in rec repair. 
  Reference    Z. Naturforsch. 44c, 791 (1989); received April 14/May 24 1989 
  Published    1989 
  Keywords    Neocarzinostatin, DNA R epair, E coli, Hypotonie Buffer, Survival Curves 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0791.pdf 
 Identifier    ZNC-1989-44c-0791 
 Volume    44