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'Dl Peptide' in keywords
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1999 (1)
1995 (1)
1Author    O. Kruse, G. H. SchmidRequires cookie*
 Title    The Role of Phosphatidylglycerol as a Functional Effector and Membrane Anchor of the D l-C ore Peptide from Photosystem II-Particles of the Cyanobacterium Oscillatoria chalybea  
 Abstract    The intrinsic polypeptide D l, isolated from photosystem (PS) Il-particles of the cyanobac­ terium Oscillatoria chalybea, was obtained by electroelution and fractionated extraction with organic solvents. Purification was demonstrated by Western blotting and amino acid sequenc­ ing. By carrying out D1 -immunization in rabbits a polyclonal monospecific D1-antiserum was obtained. For the qualitative characterization of D1 as a lipid-binding peptide, the effect of the lipids phosphatidylglycerol (PG), monogalactosyldiacylglyceride (M G D G) and phosphatidylcho­ line (PC) on PSII-oxygen evolution was analysed in reconstitution experiments. In these experiments purified photosystem II (PSII)-particle preparations were treated with the en­ zyme phospholipase A 2 and supplemented with lipid emulsions. We were able to show that the inhibition of electron transport, as the consequence of this lipase treatment, was only relieved, if phosphatidylglycerol was added to the preparation. A model was proposed, in which phosphatidylglycerol is a functional effector for the optimal conformation of D 1 in the PSII core complex. Phosphatidylglycerol molecules are unusually tightly bound to the D1 peptide by hydrophobic interactions. A covalent binding seems not probable. The localisation of phosphatidylglycerol binding sites was found by trypsin treatment of D1 and analysis of the obtained oligopeptides with HPLC and immunoblotting. The binding sites could be con­ fined to the hydrophobic amino acid section between arginine 27 and arginine 225. which is known to be the membrane anchor of D l. This has led us to the conclusion that the phospho­ lipid phosphatidylglycerol plays an important role for anchoring the Dl-peptide and for its orientation in the thylakoid membrane. Phosphatidylglycerol with its high amount of palmitic acid has in prokaryotic cyanobacteria apparently a role in stabilization and orientation. The high turn-over of D l and the spatial separation of the synthesis-and incorporation-site in the membrane, developed during evolution in eukaryotic organisms, might have changed the requirement on the mobility and the orientation of D l in photosynthetic membranes. 
  Reference    Z. Naturforsch. 50c, 380—390 (1995); received December 12 1994/February 24 1995 
  Published    1995 
  Keywords    Dl-Peptide, Phosphatidylglycerol, Lipid-Protein Binding, Cyanobacterium, PSII-Complex 
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 TEI-XML for    default:Reihe_C/50/ZNC-1995-50c-0380.pdf 
 Identifier    ZNC-1995-50c-0380 
 Volume    50 
2Author    A. Nette Gasser, Stefan Raddatz, Alfons Radunz, GeorgH. SchmidRequires cookie*
 Title    Comparative Immunological and Chemical Analysis of Lipids and Carotenoids of the D l-Peptide and of the Light-Harvesting-Complex of Photosystem II of Nicotiana tabacum  
  Reference    Z. Naturforsch. 54c, 199—208 (1999); received November 11/December 18 1998 
  Published    1999 
  Keywords    Light-Harvesting complex, Dl-Peptide, PS Il-Complex, Lipid-Peptide-Binding, Antibodies 
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 TEI-XML for    default:Reihe_C/54/ZNC-1999-54c-0199.pdf 
 Identifier    ZNC-1999-54c-0199 
 Volume    54