| | 1 | Author
| Helmut Heinle, Gerhard Sigg, Amo Reich, Klaus-Ulrich Thiedemann | Requires cookie* | | | Title
| Metabolie Effects of Direct Current Stimulation on Cultured Vascular Smooth Muscle Cells  | | | | Abstract
| Vascular smooth muscle cells from rabbit arteries were grown in tissue culture and stimulated by DC impulses (1 mA, 1 V, 10 Hz, 1 ms/imp). Scanning microscopic examination disclosed that in stimulated cultures the cell surface was enlarged by numerous microvilli. This was interpreted as being indicative of an increase in cell activity. Cellular metabolism was character ized by analyzing the incubation medium for glucose, glutamate/glutamine, and lactate. When compared to unstimulated controls, stimulation caused an increase in the uptake of glucose and glutamine as well as an increased lactate production. The enhancing effect on metabolism was prevented when the "calcium antagonist" verapamil was present (5 x 10-6 m). Although the exact mechanism by which D C stimulation influences the cells remains obscure, this finding indicates an important mediating role of Ca2+ ions. | | | |
Reference
| Z. Naturforsch. 39c, 1141—1144 (1984); received August 8 1984 | | | |
Published
| 1984 | | | |
Keywords
| Cell Culture, Direct Electric Stimulation, Metabolie Effects, Calcium Antagonist, Vascular Smooth Muscle | | | |
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| default:Reihe_C/39/ZNC-1984-39c-1141.pdf | | | | Identifier
| ZNC-1984-39c-1141 | | | | Volume
| 39 | |
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