| | 1 | Author
| Rainer Jaenicke, Hans-Dietrich Liidemann, Gerhard Schmid | Requires cookie* | | | Title
| Pressure, Temperature and pH Dependence of the Absorption Spectrum of Reduced Nicotinamide Adenine Dinucleotide  | | | | Abstract
| Enzymological studies at high hydrostatic pressure generally involve temperature, pH and pressure as variables, owing to the effect of adiabatic compression and the ionization volume o f the buffer system. In the case of N AD dependent oxidoreductases this implies that the extinction coefficient o f the coenzyme may be affected by p, T and pH, apart from the spectral change accompanying the redox reaction. Measurements o f the pressure dependence of the absorbance of N AD H show a slight red shift and a 1% decrease (3% increase) o f the absorbance at 339 nm (360 nm) at 2 kbar. The pH depen dence at the given wavelengths amounts to —(2.4 ± 0.1)% per pH unit (25 °C), while the intrinsic temperature effect (after correction for thermal expansion) is o f the order o f -0.2% per degree (2 0 -3 0 °C). Applying buffers with negligible ionization volume, 366 nm is the optimum wavelength for high pressure studies up to 2 kbar because here the pressure dependent spectral changes o f the N ADH absorption vanish. | | | |
Reference
| Z. Naturforsch. 36c, 84—8 (1981); received October 9 1980 | | | |
Published
| 1981 | | | |
Keywords
| Absorption, Dehydrogenases, High Pressure, NADH, Oxidoreductases, pH Dependence, Tem perature Dependence | | | |
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| default:Reihe_C/36/ZNC-1981-36c-0084.pdf | | | | Identifier
| ZNC-1981-36c-0084 | | | | Volume
| 36 | |
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