| | 1 | Author
| Akira Taketo | Requires cookie* | | | Title
| Sensitivity of Escherichia coli to Viral Nucleic Acid. XVI. Temperature Conditions for Ca2+-Dependent DNA Uptake in Escherichia coli  | | | | Abstract
| Properties of C a2+-or Ba2+-dependent transfection and transform ation in Escherichia coli were examined, using (PX174 replicative-form (R F) D NA and plasm id DNA. F or the transfection and transformation, a heat pulse step was dispensable and the yield o f transfectants was, in most E. coli strains, rather reduced by the heat treatment. The heat pulse step was also detrim ental for the transformation o f certain strains such as lipopolysaccharide mutants. The first stage o f the DNA uptake process (formation of DNA • recipient cell complex) was dependent on low tem perature and Ca2+ ion. A substantial am ount o f the complexed R F-D N A was released from the bacteria, by washing with a chilled Tris buffer. Although a R F-D N A • cell complex was formed even at 37 °C or in chilled 0.05 m MgCl2, the complex did not yield transfectants. | | | |
Reference
| Z. Naturforsch. 37c, 87—92 (1982); received October 141981 | | | |
Published
| 1982 | | | |
Keywords
| DNA, Transfection, Transformation, Escherichia coli | | | |
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| default:Reihe_C/37/ZNC-1982-37c-0087.pdf | | | | Identifier
| ZNC-1982-37c-0087 | | | | Volume
| 37 | |
| 2 | Author
| N. B. Rr, F. Urlong, K. Oen, M. Arien | Requires cookie* | | | Title
| Further Observations on Periodicities of Nucleotide Occurrences in Natural D N A 's | | | | Abstract
| There are non-random features in the occurrences of nucleotides in the D NA's of certain organisms which are detectable by statistical analyses of the entire sequence. Earlier, using the bacteriophage Phi-X 174 DNA sequence, we had reported that the self-information values for one type of dinucleotide association showed a marked periodicity when their autocorrelation coeffi cients were graphed. A similar, but computationally simpler, analysis has been developed which gives a comparable indication of periodicity. The difference, in average autocorrelation coeffi cients obtained with this analysis, between the peak values and all others has been used as an index to compare the extent of periodic non-randomness for a series of natural DNA sequences and for various artificial sequences. Calculations show that triplet periodicity, the relationship between dinucleotides separated by a single nucleotide, is characteristic only of the natural sequences of certain filamentous phages and is not found prominently in any other DNA analyzed (including sequences of similar length from plasmids, yeast, bacteria and higher animals). By shuffling nucleotides in a given sequence or by substituting selected nucleotides to alter various positions in both periodic and aperiodic sequences, we have found that an excess or deficiency of a given nucleotide at one of the three positions in a triplet reading frame can simulate the periodic characteristic. Thus, it appears that this global statistical analysis detects the tendency for single strand phages to utilize a specific nucleotide, rather than one randomly selected, to constitute codons. | | | |
Reference
| Z. Naturforsch. 40c, 854—857 (1985); received May 20 1985 | | | |
Published
| 1985 | | | |
Keywords
| DNA, Sequences, Autocorrelation, Periodicity, Bacteriophage | | | |
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| default:Reihe_C/40/ZNC-1985-40c-0854.pdf | | | | Identifier
| ZNC-1985-40c-0854 | | | | Volume
| 40 | |
| 3 | Author
| Regina Teschner, Stefan Postius, Monika Löffler, Friedhelm Schneider | Requires cookie* | | | Title
| Proliferation and Metabolie Activities of Ehrlich Ascites Tumor Cells in Chemically Defined Albumin Media | | | | Abstract
| , amino acid transport, respiration and lactate/glucose quotient of Ehrlich ascites tumor cells grown in suspension culture in serum free medium supplemented with albumin charges of different origin were studied. Optimal cell growth was obtained in nutrient medium supplemented with 1% bovine serum albumin (Cohn-fraction V, Serva). Cell proliferation under these culture conditions was delayed to 50% as compar ed to controls in normal medium; the rate of synthesis of macromolecules was reduced; energy metabolism was not significantly impaired. The trend of the cells in albumin medium to attach to glass was independent from the pH of the cultures between 7.2 and 8.0; it was enhanced by fatty acid deprivation of the albumin. | | | |
Reference
| Z. Naturforsch. 34c, 805 (1979); received May 16 1979 | | | |
Published
| 1979 | | | |
Keywords
| Cultured Cells, Chemically Defined Albumin Media, Cell Proliferation Cell proliferation, viability, DNA-, RNA-, protein synthesis | | | |
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| default:Reihe_C/34/ZNC-1979-34c-0805.pdf | | | | Identifier
| ZNC-1979-34c-0805 | | | | Volume
| 34 | |
| 4 | Author
| Akira Taketo | Requires cookie* | | | Title
| Ba2+-Induced Competence for Transfecting D NA | | | | Abstract
| Effect of alkaline earth metal ions on induction of the competence for DNA transfection was investigated. Unlike spheroplasts, the bulk of the bacteria treated with these ions retains colony-forming ability. The order of effectiveness for transfection of replicative-form DNA has been found to be Ba2+> C a 2+> S r 2+> M g 2+. The competence of Ba2+-treated cells is 3 to 5 times higher than that of Ca^-treated bacteria and about 40 times higher than that of lysozyme-EDTA sphero plasts. The Ba^-dependent transfection is cryophilic and formation of the infective complex occurs very rapidly at 0 °C, but not at 37 °C. | | | |
Reference
| (Z. Naturforsch. 30c, 520—522 [1975]; received March 11 1975) | | | |
Published
| 1975 | | | |
Keywords
| Ba2+, Competence, DNA, Escherichia coli, Transfection | | | |
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| default:Reihe_C/30/ZNC-1975-30c-0520.pdf | | | | Identifier
| ZNC-1975-30c-0520 | | | | Volume
| 30 | |
| 5 | Author
| W. Ilhelm Uecker, D.Iethard Kubsch, D. Ieter Lutz, Hans Kröger | Requires cookie* | | | Title
| Einbau von |32P|Orthophosphat in DNA der Mäuse-Milz unter Antigen-Einfluß Incorporation of [32P]O rthophosphate into DN A o f the Mouse Spleen under Influence o f Antigen | | | | Abstract
| Antigens cause an increase o f the D NA synthesis in the spleen o f mice as shown by the in corporation of [32P]orthophosphate and [3H]thym idine. In the present paper, the incorporation of [32P]orthophosphate into the single deoxymononucleotides has been studied under influence of the antigen bovine serum albumin. For this purpose, the labelled D NA was decomposed to the deoxymononucleotides and their specific activities determ ined. Using [32P] orthophosphate, it was found that the activity o f the D NA increased continuously during an observation period of 8 h. While after one hour the relative specific activity o f dTM P was highest and that o f dAMP lowest, the relative specific activities o f the deoxymononucleotides had become equal after 8 h. Under the influence of bovine serum album in, the incorporation o f [32P]orthophosphate increased in the main band DNA as well as in the satellite DNA. The antigen had no effect on the distribution of the specific activities o f the deoxymononucleotides. | | | |
Reference
| Z. Naturforsch. 35c, 599—604 (1980); eingegangen am 24. F ebruar/25. April 1980 | | | |
Published
| 1980 | | | |
Keywords
| Orthophosphate, Mouse Spleen, DNA, Antigen, Desoxymononucleotides | | | |
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| default:Reihe_C/35/ZNC-1980-35c-0599.pdf | | | | Identifier
| ZNC-1980-35c-0599 | | | | Volume
| 35 | |
| 7 | Author
| N. Burr Furlong, C. F. Beckner | Requires cookie* | | | Title
| Periodicities of Dinucleotide Self-Information Values in 0X 174 DNA | | | | Abstract
| The natural DNA sequence of bacteriophage, 0X174, when analyzed as a "text" of dinucleo tides, is shown to display an easily detectable degree of non-randomness by the distribution of values of dinucleotide self-information along the sequence. Self-information corresponding to occurrences of dinucleotides separated by a single nucleotide is found to be somewhat higher than the values which preceed or follow it for every third nucleotide position along the sequence. Consequently autocorrelation coefficients of these values display a strong periodicity and harmonic analysis of the values shows a spike at a value of 3. Self-information autocorrelation periodicity is used as a test of the effect of randomizing portions of the sequence. Any one or two of the three nucleotides in each triplet of the sequence can be chosen at random without losing dinucleotide self-information periodicity except when both the 1st and 3rd nucleotide of all of the triplets in the major <£X174 protein reading frame are randomized. Periodicity is also lost when sequences are generated by randomizing triplets. Autocorrelation and harmonic analysis also indicate other less marked periodic features of dinucleotide self-information values of the native sequence; non-random features are suggested at periods of 12,20 and 24 nucleotides. | | | |
Reference
| Z. Naturforsch. 37c, 321—325 (1982); received September 22 1981 | | | |
Published
| 1982 | | | |
Keywords
| DNA, Self-Information, Bacteriophage, Autocorrelation, Periodicity | | | |
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| default:Reihe_C/37/ZNC-1982-37c-0321.pdf | | | | Identifier
| ZNC-1982-37c-0321 | | | | Volume
| 37 | |
| 8 | Author
| JanuszVladimír Blasiaka, Zofia Kleinwächterb, RenataŽ. Walter3, Aludováb | Requires cookie* | | | Title
| Interaction of Organophosphorus Insecticide Methylparathion with Calf Thymus D N A and a Synthetic D N A Duplex | | | | Abstract
| The interaction of an organophosphorus insecticide m ethylparathion (O.O-dimethyl 0-4-nitrophenyl phosphorothioate) with double-stranded DNA was characterized by UV and circular dichroism (CD) spectroscopy. Two kinds of DNA were employed: calf thymus DNA (CT DNA) and a synthetic two-stranded oligomer of sequence 5'-d(TTG GA TCCG AA TT-C A A G CTT)-3\ Melting curves and CD spectra were taken for the DNAs in the presence of the insecticide at methylparathion/DNA base pair molar ratio of 0.5. The insecticide evoked a decrease of the melting tem perature and a broadening of the transition range for CT DNA. Similar effects were observed for the synthetic oligomer but they were less pronounced than in the case of CT DNA. M ethylparathion evoked a slight shift and an increase in the ampli tude of the negative band in the CD spectra of both DNAs. O btained results indicate that methylparathion may perturb the thermal stability and conformation of DNA, which is an evidence that the insecticide has an ability to interact directly with DNA. | | | |
Reference
| Z. Naturforsch. 50c, 820—823 (1995); received August 8/Septem ber 19. 1995 | | | |
Published
| 1995 | | | |
Keywords
| DNA, Methylparathion, Melting Curves, Circular Dichroism, Pesticides | | | |
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| default:Reihe_C/50/ZNC-1995-50c-0820.pdf | | | | Identifier
| ZNC-1995-50c-0820 | | | | Volume
| 50 | |
| 10 | Author
| Ingunn Ulrich, Wolfgang Ulrich | Requires cookie* | | | Title
| Flow Cytometric DNA-Analysis of Plant Protoplasts Stained with DAPI | | | | Abstract
| prepared from leaves of various higher plants were stained with the specific DNA fluorochrome DAPI and measured with a pulse cytophotometer. DNA distribution curves of protoplasts showed DNA histograms like assays of animal cells stained with the same dye solu tion. The percentage of fractions of cell populations in Go/Gr , S-and G2 + M-phase of the cell cycle could be calculated. A simple method of protoplasts preparation and of flow cytometric estimation of DAPI stained plant protoplasts is described. This method allows cytogenetic and cytokinetic studies of cells from plant material and may be used for fluorescence activated cell sorting. | | | |
Reference
| Z. Naturforsch. 41c, 1052 (1986); received July 23 1986 | | | |
Published
| 1986 | | | |
Keywords
| Flow Cytometry, Plant Protoplasts, Protoplasts Preparation, DNA, DAPI Protoplasts | | | |
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| default:Reihe_C/41/ZNC-1986-41c-1052.pdf | | | | Identifier
| ZNC-1986-41c-1052 | | | | Volume
| 41 | |
| 11 | Author
| ElenaG. Paneva3, NadejdaC. Spassovska3, KonstantinC. Grancharov3, JordankaS. Zlatanovab, JuliaN. Yaneva3 | Requires cookie* | | | Title
| Interaction of Histone H 1 with cis-Platinum Modified DNA | | | | Abstract
| Complexes Cw-diamminedichloroplatinum(II) (c « -D D P) is known as an effective anticancer drug. Its therapeutic effect is supposed to be a consequence of the covalent binding to D NA. A number of cellular proteins were found to bind selectively to D N A modified by c/s-DD P (but not by its isomer frans-D D P). Here we present our observations on interaction of the linker histone H I with cis-and frans-DDP modified DNA fragments. The | | | |
Reference
| Z. Naturforsch. 53c, 135 (1998); received Novem ber 13/D ecem ber 18 1997 | | | |
Published
| 1998 | | | |
Keywords
| cis-DDP, trans-DDP, Histone H I, DNA, Protein/DNA | | | |
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| default:Reihe_C/53/ZNC-1998-53c-0135_n.pdf | | | | Identifier
| ZNC-1998-53c-0135_n | | | | Volume
| 53 | |
| 12 | Author
| Eberhard Bothe, G. Ali, Ureshi, D. Ietrich, S. Ch, Lte-F Ro, H. Lin D | Requires cookie* | | | Title
| Rate of OH Radical Induced Strand Break Formation in Single Stranded DNA under Anoxic Conditions. An Investigation in Aqueous Solutions Using Conductivity Methods | | | | Abstract
| Single stranded D N A (ssD N A) in aqueous N 20-satu rated solution was pulse-irradiated with electrons or irradiated under steady-state conditions with 60Co-y rays. The conductivity increase after irradiation was measured as a function o f concentration, pH, temperature, metal cation content and additives. The conductivity increase could be shown to be due to the release o f associated counterions (N a+ or H+) as a result o f the formation o f chain breaks. At 28 mg I-1 D N A ~ 8 sodium ions are liberated per 100 eV absorbed energy (G (N a +) = 8.3). On the basis o f G value for single-strand breaks (ssb) o f G(ssb) = 0.55, it is calculated that 8.3/0.55 = 15 sodium ions per strand break are set free. The release o f N a+ monitored by 23N a-N M R at pH 7 as a function o f dose corresponds to that o f the conductivity increase. The rate o f the conductivity increase does not depend on d ose/p u lse (range 2 -2 0 J kg-1) and shows at least two components. The rate constant o f the fast and dom inant com ponent is constant above pH 8.5 (A: = 38 s— 1, 20 °C) and increases linearly with proton concentration below pH 7. Values o f 13kcal/m ol for the activation energy and 5 x 10" s_1 for the frequency factor were obtained at pH 7.3. Addition o f p-benzoquinone (pBQ) increases the rate constant o f the fast component proportionally to the concentration o f pBQ. From these results the rate-determining step o f the fast conductivity increase is concluded to be the splitting o f D N A radicals. The pH dependence and the m agnitude o f the activation energy agree with results from low molecular weight model com pounds for the C-4' m echanism ; this m echanism involves a heterolytic splitting o f the phosphoric acid ester bond starting from the 4' radical o f D N A . | | | |
Reference
| Z. Naturforsch. 38c, 1030—1042 (1983); received Septem ber 19 1983 | | | |
Published
| 1983 | | | |
Keywords
| DNA, Formation o f Strand Breaks, Electrical Conductivity, Pulse R adiolysis, Rate Constants | | | |
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| default:Reihe_C/38/ZNC-1983-38c-1030.pdf | | | | Identifier
| ZNC-1983-38c-1030 | | | | Volume
| 38 | |
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