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1978 (1)
1975 (1)
1Author    M. Volm, G. MerzRequires cookie*
 Title    Einfluß von Cytoplasma-und Serumfaktoren auf den [3H]Thymidintriphosphat-Einbau isolierter Leberzellkerne Effect of Cytoplasmic and Serum Factors on the Uptake of [3H]Thymidine- triphosphate (TTP) by Isolated Nuclei of Liver Cells  
 Abstract    The actions of cytoplasm (ultracentrifuged supernatant 105 000 x g) and of serum on the DNA synthesis (uptake of [3H ]T T P) by isolated nuclei of liver cells were studied. Cytoplasm from liver cells obtained at various intervals after partial hepatectomy revealed a variable effect only with isolated nuclei of liver cells from partially hepatectomized rats. Cytoplasm obtained from liver cells eight hours after p artial hepatectomy failed to show the usual inhibitory effect. Presum ably the inhibitory substances norm ally present in cytoplasm are inactivated within a certain time after partial hepatectomy. Serum of partially hepatectomized anim als enhanced the uptake of [3H ]T T P by nuclei of normal liver cells and those from partially hepatectom ized animals. The stim ulating effect was present only in the serum obtained between four and twelve hours after partial hepatectomy. It remains unclear whether the effects described are growth factors specific for the liver. 
  Reference    (Z. Naturforsch. 30c, 107 [1975]; eingegangen am 10. Oktober 1974) 
  Published    1975 
  Keywords    DNA-Synthesis, Isolated Liver N uclei, Cytoplasm, Serum, P artial Hepatectomy 
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 TEI-XML for    default:Reihe_C/30/ZNC-1975-30c-0107.pdf 
 Identifier    ZNC-1975-30c-0107 
 Volume    30 
2Author    Klaus Scheller, Peter Karlson, Dietrich BodensteinRequires cookie*
 Title    Effects of Ecdysterone and the Juvenile Hormone Analogue Methoprene on Protein, R N A and D N A Synthesis in Wing Discs of Calliphora vicina  
 Abstract    Adolf Butenandt zum 75. G ebu rtstag gew idm et The protein, RNA and DNA content of wing discs increase exponentially during the last larval instar. Biosynthesis of protein, RNA and DNA was studied by injecting labelled precursors into larvae or white prepupae and measuring the incorporation in the wing discs dissected out after an appropriate time. Protein synthesis is stimulated by ecdysterone, methoprene has no effect. Bio­ synthesis of rRNA is increased in wing discs of white prepupae after ecdysterone or methoprene injection. Methoprene inhibits the synthesis of mRNA, while ecdysterone has no clear-cut effect within the limits of our method (gel electrophoretic analysis). Ecdysterone and methoprene have no detectable influence on incorporation of thymidine into DNA, but the incorporation label from uridine into DNA is diminished; this effect may be due to changes in the precursor pool. 
  Reference    Z. Naturforsch. 33c, 253 (1978); received March 9 1978 
  Published    1978 
  Keywords    Ecdysterone, Juvenile Hormone, Wing Discs, Protein Synthesis, RNA Synthesis, DNA Synthesis, C alliph ora vicina 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0253.pdf 
 Identifier    ZNC-1978-33c-0253 
 Volume    33