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1999 (1)
1988 (1)
1Author    Kaeko Kikuchi, Yoshio Taniyama, Ryuji MarumotoRequires cookie*
 Title    Evaluation of the 2 NH 2 A—T Pair in Hybridization, I Synthesis of the DNA/RNA Hybrid Oligomers Containing 2-Aminoadenosines  
 Abstract    DNA decamers containing 2-aminoadenosine were synthesized. Oligonucleotide duplexes in-cluding the 2 NH2A—T base pairs were prepared and their Tm profile examined. Contrary to expectation, elevation of the Tm value by the 2 NH2 group is very small in DNA/RNA duplexes. From the CD spectra measurement, we assume that the distortion of the B-DNA structure caused by scattered DNA/RNA base pairing diminishes the efficient hydrogen bonding and base stacking of the duplexes. It was also found that the DNA duplexes containing 2-aminoadenosine hybrids are considerably resistant to ribonuclease T2 or nuclease PI digestion. 
  Reference    Z. Naturforsch. 43b, 623—630 (1988); received October 12 1987/February 1 1988 
  Published    1988 
  Keywords    DNA Probe, Hybridization, 2-Aminoadenosine, CD Spectra, DNA/RNA Hybrid Oligomer 
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 TEI-XML for    default:Reihe_B/43/ZNB-1988-43b-0623.pdf 
 Identifier    ZNB-1988-43b-0623 
 Volume    43 
2Author    Dirk Redecker, InkeS. Feder, Pablo Vinuesa, Thomas Batinic, Ulrike Schulz, Kerstin Kosch, Dietrich WernerRequires cookie*
 Title    Genotypic Characterization by DNA Fingerprinting  
 Abstract    A DNA probe specific for biocontrol strains of Pseudomonas was produced by screening randomly amplified polymorphic DNA (R A P D) PCR fragments. Specificity of the probe was assessed by dot blot and colony hybridization. It was used to specifically determine the population of these strains on roots of Cucumis sativus cv. Delikatess. Two polymorphic R A PD fragments of 750 bp, and 550 bp showed identical specificity. The biocontrol strain Pseudomonas sp. W34 was shown to be competitive in the rhizosphere of cucumber and to maintain a stable population for at least 10 days when inoculated on the seed. The phylogenetic relationships between the biocontrol and reference strains were analyzed at the strain level by means of R A PD and repetitive sequence-based PCR genomic finger­ printing (rep-PCR), and at higher taxonomic levels by means of amplified 16S ribosomal DNA restriction analysis A R D R A . It was shown that the antagonistic strains are closely related, forming a separate cluster from other non-antagonistic and reference Pseudomonas strains, their taxonomic placement remaining uncertain. 
  Reference    Z. Naturforsch. 54c, 359 (1999); received December 10 1998/January 27 1999 
  Published    1999 
  Keywords    Pseudomonas sp W34, Biocontrol, Cucumis sativus, DNA Fingerprinting, DNA Probe 
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 TEI-XML for    default:Reihe_C/54/ZNC-1999-54c-0359.pdf 
 Identifier    ZNC-1999-54c-0359 
 Volume    54