| | 1 | Author
| Dirk Redecker, InkeS. Feder, Pablo Vinuesa, Thomas Batinic, Ulrike Schulz, Kerstin Kosch, Dietrich Werner | Requires cookie* | | | Title
| Genotypic Characterization by DNA Fingerprinting  | | | | Abstract
| A DNA probe specific for biocontrol strains of Pseudomonas was produced by screening randomly amplified polymorphic DNA (R A P D) PCR fragments. Specificity of the probe was assessed by dot blot and colony hybridization. It was used to specifically determine the population of these strains on roots of Cucumis sativus cv. Delikatess. Two polymorphic R A PD fragments of 750 bp, and 550 bp showed identical specificity. The biocontrol strain Pseudomonas sp. W34 was shown to be competitive in the rhizosphere of cucumber and to maintain a stable population for at least 10 days when inoculated on the seed. The phylogenetic relationships between the biocontrol and reference strains were analyzed at the strain level by means of R A PD and repetitive sequence-based PCR genomic finger printing (rep-PCR), and at higher taxonomic levels by means of amplified 16S ribosomal DNA restriction analysis A R D R A . It was shown that the antagonistic strains are closely related, forming a separate cluster from other non-antagonistic and reference Pseudomonas strains, their taxonomic placement remaining uncertain. | | | |
Reference
| Z. Naturforsch. 54c, 359 (1999); received December 10 1998/January 27 1999 | | | |
Published
| 1999 | | | |
Keywords
| Pseudomonas sp W34, Biocontrol, Cucumis sativus, DNA Fingerprinting, DNA Probe | | | |
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| default:Reihe_C/54/ZNC-1999-54c-0359.pdf | | | | Identifier
| ZNC-1999-54c-0359 | | | | Volume
| 54 | |
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