| | 1 | Author
| G. Ünter, F. W. Ildner, Claudia Fiebig, N. Orbert Dedner, H. E. Meyer | Requires cookie* | | | Title
| The Use of HPLC for the Purification of the QB-Protein  | | | | Abstract
| The 0 B-protein is as a hydrophobic integral membrane protein firmly bound in the reaction center com plex o f photosystem II. A new method was developed to purify the SD S extracted protein using reversed-phase chromatography with two binary linear gradient systems. The iden tification o f the 0 B-protein was achieved by its rapidly labeling during photoassim ilation of [35S]sulfate and by its reaction with the photoaffinity label azido-[14C]atrazine. Furtherm ore, antisera against the purified Q B-protein reacted with a single peak fraction, the second peak o f the chrom atogram , which was identical with the labeled protein peak fraction. | | | |
Reference
| Z. Naturforsch. 42c, 739 (1987); received N ovem ber 10 1986 | | | |
Published
| 1987 | | | |
Keywords
| H PLC-Protein Purification, Q B-Protein, D -l Protein, psbA G ene, Chlamydomonas reinhardii | | | |
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| default:Reihe_C/42/ZNC-1987-42c-0739.pdf | | | | Identifier
| ZNC-1987-42c-0739 | | | | Volume
| 42 | |
| 2 | Author
| Jack Dekker, RonaldG. Burmester | Requires cookie* | | | Title
| Differential Pleiotropy in a psbA Gene Mutant of Brassica napus Implies Altered Temporal Photosynthesis and Thermal Tolerance | | | | Abstract
| Studies were conducted to test the hypothesis that the mutation to the psbA. plastid gene that confers s-triazine resistance (R) also results in an altered diurnal pattern o f photosynthetic car bon reduction (CER) relative to that o f the susceptible (S) wild-type. In all experiments CER approximated the increasing and decreasing light levels during the diurnal. S C ER exceeded that o f R during the midday period, but R CER was greater early and late in the diurnal at 25 °C. R CER exceeded that o f S for most o f the diurnal at 35 °C and in older, crowded, nitrogen-starved plants. These studies support the stated hypothesis and indicate a more complex model o f photosynthetic productivity than previously observed. An assessment o f the photosynthetic competence o f either biotype may be a function o f the time o f day or the environmental condi tions the plants are exposed to, especially temperature. | | | |
Reference
| Z. Naturforsch. 45c, 474 (1990); received October 18 1989 | | | |
Published
| 1990 | | | |
Keywords
| Photosystem II, D -l Protein, Electron Transport, C hronobiology, Diurnal Rhythms | | | |
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| default:Reihe_C/45/ZNC-1990-45c-0474.pdf | | | | Identifier
| ZNC-1990-45c-0474 | | | | Volume
| 45 | |
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