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1982 (1)
1Author    K. Grossmann, H. Bisswanger, H. U. SeitzRequires cookie*
 Title    Differential Fluorescence and Kinetic Studies on the Template-Binding of RNA Polymerase from Parsley and Escherichia coli  
 Abstract    A fluorescence spectroscopy m ethod is described for studying association o f RNA polymerase with DNA templates. Using double beam differential fluorescence at excitation and emission wavelengths of 285 and 335 nm, respectively, the new technique discriminates non-specific decrease of fluorescence intensity by addition o f D N A from quenching of polymerase fluorescence by protein-nucleic acid interactions. C om paring the results with studies of UM P incorporation into RNA, the Äg-values o f tem plate-binding were in good agreem ent with the values for RNA synthesis, pointing to specific interaction o f polym erase and the D NA tem plate measured by the fluorescence method. W hile E. coli enzyme showed higher affinity for tem plates such as heat-denatured poly [d(A-T)] and poly [d(G -C)] parsley RNA polym erase I accepted such templates with the same affinity as salmon sperm DNA. It is obvious that divalent cations are not necessary for the interaction of both enzymes with single-stranded DNA -tem plates. UM P incorporation studies suggest that transcription is a cooperative process. 
  Reference    Z. Naturforsch. 37c, 81—86 (1982); received O ctober 261981 
  Published    1982 
  Keywords    RNA Polymerase, Template-Binding, D ifferential Fluorescence, Parsley, E coli 
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 TEI-XML for    default:Reihe_C/37/ZNC-1982-37c-0081.pdf 
 Identifier    ZNC-1982-37c-0081 
 Volume    37