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'D 1 Protein' in keywords Facet   Publication Year 1993  [X]
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1Author    Walter Oettmeier, Klaus Masson, Ralf Kloos, Ellen ReilRequires cookie*
 Title    On the Orientation of Photosystem II Inhibitors in the QB-Binding Niche: Acridones, Xanthones and Quinones  
 Abstract    The orientation o f acridones, xanthones, 1,4-benzo-and naphthoquinones within the ph o­ tosystem II Q b herbicide-binding niche was studied by means o f mild trypsination and by esti­ mation o f p /50-values in Chlamydomonas reinhardtii D 1 mutants (Val219 > lie, A la25, > Val, Phe255 > Tyr, Ser264 > Ala, A sn266 > Thr, and Leu275 > Phe). A s judged from the R/S-values (ratios o f -^50 -values resistant versus susceptible type) close to 1 in all mutants, the acridones and xanthones do not have strong interactions with the parent amino acids. Contrary, the qui-nones exhibit extreme low R/S-values down to 0.003 (for 2,5-dibrom o-3-m ethyl-6-isopropyl-1,4-benzoquinone; D BM IB) in the Ser264 mutant. This extreme negative cross resistance or supersensitivity indicates that the quinones do not droxyl group. 
  Reference    Z. Naturforsch. 48c, 146—151 (1993); received December 3 1992 
  Published    1993 
  Keywords    Chlamydomonas reinhardtii Mutants, D 1 Protein, Antibody, Trypsination 
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 TEI-XML for    default:Reihe_C/48/ZNC-1993-48c-0146.pdf 
 Identifier    ZNC-1993-48c-0146 
 Volume    48 
2Author    KlausG. Tietjen, Wilfried Draber, John Goossens, JohannesR. Jansen, JoachimF. Kluth, Michael Schindler, Heinz-Jürgen, Wroblowsky Bayer, A. G.Requires cookie*
 Title    Binding of Triazines and Triazinones in the QB-Binding Niche of Photosystem II  
 Abstract    A series o f 20 triazines (derivatives o f 2-alkylam ino-4-benzylam ino-6-chloro-l,3,5-triazines) and 37 triazinones (derivatives o f 3-alkyl-4-am ino-6-phenyl-l,2,4-triazin-5-ones) is tested for inhibitory potency in photosynthetic electron flow through photosystem II o f wild type Chla-mydomonas reinhardtii and o f five mutants with aminoacid substitutions in the D 1 protein at valine 219, alanine 251, phenylalanine 255, serine 264, and leucine 275. The data are used for computer modelling o f .the possible location o f the com pounds within a three dimensional model o f the QB-binding niche o f the D 1 protein. 
  Reference    Z. Naturforsch. 48c, 205 (1993); received November 23 1992 
  Published    1993 
  Keywords    D 1 Protein, Herbicides, Molecular M odeling, Photosystem II, Q B-Binding Niche 
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 TEI-XML for    default:Reihe_C/48/ZNC-1993-48c-0205.pdf 
 Identifier    ZNC-1993-48c-0205 
 Volume    48 
3Author    Aloysius Wild, Petra Strobel, Ute FlammersfeldRequires cookie*
 Title    Studies of Components of the Thylakoid Membrane of Undamaged and Damaged Spruce Trees at Different Mountain Sites  
 Abstract    During a five-year period, com ponents o f the thylakoid membrane in needles o f the second generation o f undamaged and damaged trees o f Norway spruce were studied at three different mountain sites in W est Germany. Visible signs o f damage at these sites are a yellowing o f the light-exposed sides o f the needles as well as the loss o f needles. The goal o f this study was to determine damage-induced alterations in com position and physiological reactions o f the thy­ lakoid membranes in spruce needles. In order to meet this purpose, contents o f chlorophyll a and b, electron transport rate o f photosystem II, contents o f the D 1 protein, cytoch rom e/, as well as P-700 were measured. The chlorophyll content in the needles o f the damaged spruce trees was significantly lower than in the needles o f the undamaged trees. In addition to this, the typical annual course o f chlorophyll content was exclusively observed in the needles o f the undamaged spruce trees. If related to dry weight, a drastic reduction o f the electron transport rate and o f the redox com ­ ponents o f the thylakoid membrane was observed due to damage, indicating a degeneration o f the photosynthetic membranes. The contents o f D 1 protein and the photosynthetic electron transport rates were also markedly reduced in the needles o f the damaged trees, when related to chlorophyll content o f thylakoids, suggesting an early and particular impairment o f photo­ system II. The comparison o f spruce trees showing different signs o f damage demonstrates that certain biochemical parameters concerning the photosynthetic membranes (chlorophyll, cytochrom e/ , ratio photosystem II/I) reflect the extent o f damage and are suitable for an early indication o f a beginning, but still invisible damage o f spruce trees. 
  Reference    Z. Naturforsch. 48c, 911 (1993); received June 5/August 27 1993 
  Published    1993 
  Keywords    Chlorophyll, Cytochrome /, D 1 Protein, Forest Decline, P-700, Photosynthetic Electron Transport 
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 TEI-XML for    default:Reihe_C/48/ZNC-1993-48c-0911.pdf 
 Identifier    ZNC-1993-48c-0911 
 Volume    48