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1986 (1)
1981 (1)
1979 (1)
1Author    Peter Dancker, Agnes KlicheRequires cookie*
 Title    Cytochalasin B-Induced ATPase Activity of Actin: Dependence on Monomer Concentration  
 Abstract    We simultaneously measured polymerization and ATPase activity of actin induced by cytochalasin B. It was found that under all conditions tested, ATPase activity was proportional to the concentration of actin which was unpolymerized. In addition, it was found that conditions increasing ATPase activity also increase the velocity o f polymerization. From this we conclude that CB-induced ATPase activity is a property o f the actin monomers and that the "readiness" of the monomers to hydrolyze ATP is correlated with an increased capacity o f the monomers to polymerize. 
  Reference    Z. Naturforsch. 36c, 1050—1055 (1981); received June 19 1981 
  Published    1981 
  Keywords    Actin, Actin Polymerization, Cytochalasin B 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-1050.pdf 
 Identifier    ZNC-1981-36c-1050 
 Volume    36 
2Author    B. Ernd, F. Ussm, Ann, P. Ete, D. AnRequires cookie*
 Title    Polymerization of Actin in the Absence and Presence of Cytochalasin B: Problems of Determining "Critical Concentration"  
 Abstract    A Various concentrations o f actin (0.3 or 1 mM MgCl2, 1 mM A T P , 1 mM E G T A) reached their final degree o f polym erization (m easured by a pyrene dye attached to actin) earlier in the pres­ ence o f cytochalasin B than in its absence. The curves relating concentrations o f polym eric F-actin to total actin concentration were under these conditions highly nonlinear making an unam biguous extrapolation to zero F-actin concentration (to deduce the "critical concentration" o f actin poly­ m erization) im possible. The concentration o f actin, above which polym erization occurred, was unaltered by cytochalasin B (although for reasons not yet understood the specific fluorescence intensity of polym erized actin was lower in the presence of cytochalasin B than in its absence). The results show that a distinct "critical concentration" o f actin polymerization must not always be well defined. 
  Reference    Z. Naturforsch. 41c, 781 (1986); received April 23 1986 
  Published    1986 
  Keywords    ctin, Cytochalasin B, Critical Concentration, Fluorescence, Polymerization 
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 TEI-XML for    default:Reihe_C/41/ZNC-1986-41c-0781.pdf 
 Identifier    ZNC-1986-41c-0781 
 Volume    41 
3Author    P. Dancker, I. LowRequires cookie*
 Title    Complex Influence of Cytochalasin B on Actin Polymerization  
 Abstract    In the presence of very low concentrations (about 2 x 10~7 m) of cytochalasin B (CB) the time course of actin polymerization is much more sigmoidal when followed by viscosity measurements than when followed by light scattering measurements. This suggests that under these conditions actin polymers do not immediately reach their final length but only via short "bent" polymers which can be detected only by light scattering but not by viscosity measurements. At higher CB concentrations (about equimolar to those of actin) CB reduces the average degree of polymeriza­ tion and favors the nucleation step necessary for polymerization. 
  Reference    Z. Naturforsch. 34c, 555—557 (1979); received March 13 1979 
  Published    1979 
  Keywords    Actin, Cytochalasin B, Cell Motility, Muscle Proteins, Micro filaments 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0555.pdf 
 Identifier    ZNC-1979-34c-0555 
 Volume    34