| | 1 | Author
| Z. Schweiz, Naturforsch | Requires cookie* | | | Title
| Biosynthesis of Cytochalasans. XI. New Results on the Incorporation of Phenylalanine into Cytochalasin D by Zygosporium masonii [1]  | | | | Abstract
| Incorporation of L-[2-:H]phenyl-[2-:H]alanine and L-phenyl-[2-l3C, l:,N]alanine into cytochala sin D by Zygosporium masonii involved the complete loss of both the a -;H-and the a-'^N-atom. Incorporation of a mixture of L-phenyl-[l;>N]alanine and L-[U-l4C]phenylalanine into cytochalasin D and protein amino acids (phenylalanine, leucine, isoleucine) was accompanied by a substantial loss of 15N with respect to 14C. These effects are attributed to rapid exchange reactions taking place while L-phenylalanine is part of the intracellular pool of amino acids. In addition, the medium-and concentration-dependent incorporation of the carbon skeleton of exogeneous D-phenylalanine into cytochalasin D is reported. In a peptone-based complex medium, D-phenyl-alanine is poorly incorporated. Throughout the whole concentration range (0 —250 mg/1), the incorporation rates are less than 10% of those of L-phenylalanine. In a minimal medium contain ing NH 4 NO3 as nitrogen source however, D-phenylalanine is preferred over the natural enantiomer by a factor of 1.28 up to 6.78, depending on the concentrations of exogeneous d -and L-phenylalanine. These effects are attributed to the medium-dependent activities of different amino acid transport systems responsible for the uptake of d -and L-phenylalanine in Z. masonii. | | | |
Reference
| Z. Naturforsch. 44c, 19 (1989); received September 7 1988 | | | |
Published
| 1989 | | | |
Keywords
| Cytochalasans, Cytochalasin D, Biosynthesis, Phenylalanine, Zygosporium masonii | | | |
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| default:Reihe_C/44/ZNC-1989-44c-0019.pdf | | | | Identifier
| ZNC-1989-44c-0019 | | | | Volume
| 44 | |
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