| | 2 | Author
| JonathanE. Poulton, Sun-In Shin | Requires cookie* | | | Title
| Prunasin Biosynthesis by Cell-Free Extracts from Black Cherry (Prunus serotina Ehrh.) Fruits and Leaves  | | | | Abstract
| Immature fruits and leaves o f black cherry (Prunus serotina Ehrh.) accumulate the cyanogenic glucoside prunasin (the /?-glucoside of (/?)-mandelonitrile). Cell-free extracts from these tissues catalysed the stereospecific glucosylation of (/?,S)-mandelonitrile to (/?)-prunasin at rates of 0.2 — 2.0 ^mol/h/mg protein. Uridine diphosphate glucose (ATm = 0.32rnM) acted as glucose donor. The optimum concentration of (/?,S)-mandelonitrile was 20 mM. Highest activity was exhibited at pH 7.0 —8.0 in Tris-phosphate buffer, and no additional cofactors were required. /?-Mercaptoethanol (14.5 mM), provided in the homogenization medium to prevent browning of homogenates, did not stimulate the rate o f prunasin production. In addition to (/?)-mandelo-nitrile (100%), significant activity was shown towards mandelamide (21%), benzyl alcohol (15%), mandelic acid (8%) and benzoic acid (153%), but not towards prunasin. Mandelonitrile glucosyltransferase activity was most stable at -20 °C in the presence o f 10% glycerol. | | | |
Reference
| Z. Naturforsch. 38c, 369—374 (1983); received January 3 1983 | | | |
Published
| 1983 | | | |
Keywords
| Black Cherry, Prunus serotina, Prunasin Biosynthesis, Cyanogenic Glycosides, O-Glucosyltransferase | | | |
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| default:Reihe_C/38/ZNC-1983-38c-0369.pdf | | | | Identifier
| ZNC-1983-38c-0369 | | | | Volume
| 38 | |
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