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'Cyanobacterium' in keywords Facet   Publication Year 1994  [X]
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1Author    Ulrike Strohmeier, ChristianG. Erdes3, Wolfgang LockaubRequires cookie*
 Title    Proteolysis in Heterocyst-Forming Cyanobacteria: Characterization of a Further Enzyme with Trypsin-Like Specificity, and of a Prolyl Endopeptidase from Anabaena variabilis  
 Abstract    of the cyanobacterium Anabaena variabilis ATCC 29413 and an en­ gineered mutant that lacks an intracellular protease cleaving after Lys and Arg (Maldener, Lockau, Cai, and Wolk, Mol. Gen. Genet. 225, 113-120 (1991)) were separated by ion ex­ change chromatography, and protease profiles determined using azocasein, Na-benzoyl-D,L-arginine-4-nitroanilide and N-carbobenzoxy-glycyl-L-proline-4-nitroanilide as substrates. A second enzyme cleaving at the carboxyl side of lysine and arginine, and a prolyl endopepti­ dase were detected, enriched and characterized. Both proteolytic enzymes appear to be located in the periplasm. 
  Reference    Z. Naturforsch. 49c, 70—78 (1994); received September 29/December 81993 
  Published    1994 
  Keywords    Anabaena variabilis, Cyanobacterium, Heterocysts, Proteolytic Enzymes, Prolyl Endopeptidase Soluble extracts 
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 TEI-XML for    default:Reihe_C/49/ZNC-1994-49c-0070.pdf 
 Identifier    ZNC-1994-49c-0070 
 Volume    49 
2Author    O. Kruse, A. Radunz, G. H. SchmidRequires cookie*
 Title    Phosphatidylglycerol and ß-Carotene Bound onto the D 1-Core Peptide of Photosystem II in the Filamentous Cyanobacterium Oscillatoria chalybea  
 Abstract    -particles from the cyanobacterium Oscillatoria chalybea were isolated by fractionating centrifugation. Purification of these particles was achieved by a 22 hours cen­ trifugation over a linear sucrose density gradient at 217.500 X g. The obtained particle frac­ tion exhibited an oxygen evolution activity which corresponded to three times the rate of intact cells and to five times the rate of intact thylakoids. The chlorophyll protein ratio was 1:10 and the ratio manganese/chlorophyll 1:34. SDS-polyacrylamide gel electrophoresis showed that the photosystem Il-fraction is com­ posed of the core peptides D 1 and D2, the chlorophyll-binding peptides CP 43 and CP 47, the extrinsic 33 kDa peptide (manganese stabilizing peptide, MSP) and phycobiliproteins with molecular masses between 16 to 20 kDa. Cyt b559 was not detected in our gel electro­ phoresis assay. Part of the peptides of the 30 kDa-region (D 1, D 2, MSP) occurred as aggre­ gates with a molecular mass of 60 to 66 kDa. The D 1-peptide was isolated from the PS Il-preparation by SDS-gel electrophoresis. The intrinisic peptide reacts in the Western blot procedure with the antiserum to phosphatidyl­ glycerol and with the antiserum to ß-carotene. Incubation of the peptide with the antisera to monogalactosyldiglyceride, sulfoquinovosyldiglyceride and zeaxanthine resulted negatively. The binding of phosphatidylglycerol onto the D 1-peptide was confirmed by lipid analysis in HPLC and fatty acid analysis by gas chromatography. Only this lipid, respectively the typi­ cal fatty acid mixture of this lipid was detected. The lipid is characterized by the fact that the hexadecenoic acid does not exhibit rraws-configuration, as is true for phosphatidylglycerol of higher plants and algae, but occurs in cw-configuration. With the antibody being directed towards the glycerol-phosphate residue and not towards the fatty acids, it can be concluded from the reaction of the antibodies with the bound lipid that the lipid is bound to the peptide via the fatty acid. The negatively charged phosphatidyl­ glycerol increases the hydrophobicity of the peptide and leads to a negatively charged sur­ face favouring binding of cations like calcium and magnesium. The fact that incubation of this PS Il-fraction with phospholipase inhibits photosynthetic activity by 25% which can be fully restored by addition of phosphatidylglycerol, shows that bound phosphatidylglycerol has a functional role. 
  Reference    Z. Naturforsch. 49c, 115—124 (1994); received July 16/October 181993 
  Published    1994 
  Keywords    D 1-Peptide, Phosphatidylglycerol, ß-Carotene, Antibody, Lipid-Protein Binding, Cyanobacterium, Manganese Stabilizing Peptide Photosystem II 
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 TEI-XML for    default:Reihe_C/49/ZNC-1994-49c-0115.pdf 
 Identifier    ZNC-1994-49c-0115 
 Volume    49